山西农业大学学报（自然科学版）2016，Vol.36Issue(10)：740-744,5.
大曲中高产糖化酶菌株的筛选及环境耐受性分析
Isolation of high amyloglucosidase-producing strains from Daqu and their environmental tolerance abil-ities
摘要
Abstract
Obj ective]Aboriginal mould strains with high ability to produce amyloglucosidase isolated from Daqu can be reinforced into Daqu again to lower Daqu consumption and increase the utilization rate of the material during vinegar fermentation and and improve the product quality.[Methods]The alcoholic fermenting matrix fermented for 24 hours was treated and cultured on Martin medium for mould isolation.Preliminary and second-time screening work was car-ried out according to the size of the transparent circle on the medium and the measured enzymatic activity.Environmen-tal tolerant ability were evaluated through growth mass.[Results]Preliminary screening obtained 10 mould strains that can produce amyloglucosidase.The results of enzyme activity suggested that M2、M5、M6、M8 and M1 5 were superior to the well-known high amyloglucosidase producing strain AS3.4309(M0).Their enzyme activity(U·mL-1 )were M6 (3912.08(32.11)> M5 (1413.16 (13.92)>M8(1233.63(71.07)>M15(1152.47(21.96)>M2(923.86(22.421 )>M0(828.88(22.23).Tolerant ability analysis showed that all the 5 strains were tolerant to 6% ethanol concentration, grew well at pH3.5,some even survived pH2.0,could grow at 15~30 ℃.[Conclusion]The obtained 5 mould strains has higher ability to produce amyloglucosidase than the well-known and widely used AS3.4309 and can survive the wholeprocess of Shanxi aged vinegar fermentation.关键词
山西老陈醋/酒醅/高产糖化酶菌株/环境耐受性Key words
Shanxi Aged Vinegar/Fermented matrix/High glucoamylase-producing strains/Environmental tolerance分类
生物科学引用本文复制引用
张琳,张也,王如福,霍乃蕊..大曲中高产糖化酶菌株的筛选及环境耐受性分析[J].山西农业大学学报（自然科学版）,2016,36(10):740-744,5.基金项目
山西省科技重点研发(指南)项目(2015-TN-10-2) （指南）
863计划项目(2011AA1009040102) （2011AA1009040102）
