陕西医学杂志2016，Vol.45Issue(10)：1275-1278,4.DOI:10.3969/j.issn.1000-7377.2016.10.001
白藜芦醇抑制胰腺癌裸鼠移植瘤生长的实验研究
The tumor inhibition study of resveratrol on pancreatic cancer cell xenograft in nude mice
摘要
Abstract
Objective:To investigate the antitumor effect of Resveratrol on pancreatic cancer Miapaca‐2 cell xenograft on athymic .Methods:The pancreatic cancer Miapaca‐2 cell xenograft model in nude mice was estab‐lished and randomly divided into the blank group ,the Res group and the saline negative control group .After inter‐vention with Res ,the mice was killed .The weight and volume of tumor were recorded .The pathological change of tumor tissue in each group was observed under microscope with HE staining .Real‐time quantitative RT‐polymerase chain reaction and western bolt assay were used to confirmed the expression of Bcl‐2 and Bax、p53 in tumor tissue at mRNA and protein lever .Results :The weight and volume of tumor in Res group were less than those in the negative control group(P<0 .01) .RT‐PCR and Western blot assay indicated Resveratrol of three different concentrations reg‐ulated the protein and mRNA expression of Bcl‐2 and Bax、p53 in tumor tissue .At the same time ,the expression levels gradually changed as the concentration increased ,the difference was significant (P<0 .01) .Conclusion:Res‐veratrol inhibited the growth of pancreatic cancer Miapaca‐2 cells xenograft .The effect may be related to the regula‐ting of Bcl‐2 and Bax、p53 at mRNA and protein lever .关键词
胰腺肿瘤肿瘤移植大鼠,裸肿瘤抑制蛋白/白藜芦醇Key words
Pancreatic neoplasms/Neoplasm transplantation Rats ,nude/Tumor suppressor proteins Resveratrol分类
医药卫生引用本文复制引用
秦勇,马清涌,党晓燕,孙师元..白藜芦醇抑制胰腺癌裸鼠移植瘤生长的实验研究[J].陕西医学杂志,2016,45(10):1275-1278,4.基金项目
陕西省科技攻关项目 （）
