天津中医药2016，Vol.33Issue(10)：610-613,4.DOI:10.11656/j.issn.1672-1519.2016.10.09
化痰通络法对rt-PA溶栓后急性脑梗死大鼠神经细胞凋亡及Caspase-12表达的影响
Effect on apoptosis of neural cell and Caspase-12 expression in rat cortex accepted Huatan Tongluo formula with thrombolysis pherapy after acute cerebral infarction
摘要
Abstract
[Objective] To observe the effect of Huatan Tongluo therapy on the expression of Caspase-12 protein, mRNA and apoptosis of nerve cells in the late of endoplasmic reticulum stress (ERS) in rat cortex accepted thrombolysis therapy with recombinant tissue-type plasminogen activator (rt-PA) after the acute cerebral infarction. [Methods] The 160 healthy SD rats which were randomly divided into sham group, model group, rt-PA group and experimental group. By using the MCAO rat model, the rats in rt-PA group and experimental group were injected rt-PA (concentration of 5.67 mg/kg), and those of experiment group were given Chinese herb of Huatan Tongluo therapy (concentration of 7.2 g/kg), 2 times a day , and each group was observed at four time points:6 h, 1 d, 3 d and 7 d. And then respectively observe the gene expression of Caspase-12 protein and mRNA, and the apoptosis in rats’brain neurons. [Results] Compared with sham group, the expression of Caspase-12 protein and mRNA increased significantly (P<0.05). Compared with model group, the expression of Caspase-12 protein and mRNA increased significantly (P<0.05) except 7 d which had no significant difference (P>0.05). Compared with rt-PA group, the expression levels of Caspase-12 mRNA of experiment group at 1 d and 7 d were significantly lower, and the protein had no significant difference with others (P>0.05), but with down trend. [Conclusion] Huatan Tongluo therapy can reduce the activation of Caspase-12 protein and mRNA in ERS, and inhibit the apoptosis of nerve cells, prevent reperfusion injury after acute cerebra accepted thrombolysis.关键词
化痰通络法/急性脑梗死/溶栓/凋亡途径/Caspase-12Key words
Huatan Tongluo therapy/acute cerebral infarction/thrombolysis/apoptosis pathway/Caspase-12分类
医药卫生引用本文复制引用
刘爽,石欢,周震..化痰通络法对rt-PA溶栓后急性脑梗死大鼠神经细胞凋亡及Caspase-12表达的影响[J].天津中医药,2016,33(10):610-613,4.基金项目
国家自然科学基金项目（81273942）；天津市应用基础及前沿技术研究计划（12JCZDJC25300）；天津市教委“十二五”创新团队培养计划资助（NO.TD12-5035）。 （）
