摘要
Abstract
[Objective] To investigate the effects and mechanism of astragalus on mesentery in ischemia-reperfusion rats. [Methods] The 120 excremental rats were randomly divided into six groups:sham operation group, model group, Ginkgo biloba extract (GB, 3.15 mg/kg) pretreatment group and astragalus (5, 10, 20 g/kg) pretreatment groups. Severn days before surgery, the drugs were given by intraperitoneal injection, once a day. The rat models were made by clipping superior mesenteric artery. Two hours later, the appearance of mesentery tissue was observed and the water content was detected. The histopathological changes of mesentery tissue was observed by HE staining and the Chiu’s score was detected, the activity of SOD, CAT and the contents of MDA in mesentery tissue were detected. The levels of CRP, TNF-α, IL-1β, IL-6 and IL-10 in mesentery tissue were detected. Tthe apoptosis of mesentery cells was observed by TUNEL staining and the apoptosis index (AI) was calculated. The activity of Caspase-3 and Caspase-9 in mesentery tissue were detected, and the expression of NF-κB was detected by Western Blot. [Results] Compared with model group, the appearance of mesentery tissue in astragalus pretreatment groups were improved, and the water content in astragalus (10, 20 g/kg) pretreatment groups were significantly decreased The histopathological changes of mesentery tissue in astragalus pretreatment groups were significantly improved. The Chiu’s score in astragalus (10, 20 g/kg) pretreatment groups were significantly decreased. The activity of SOD and CAT in mesentery tissue was significantly increased and the contents of MDA were significantly decreased. The level of CRP, TNF-αand IL-6 in mesentery tissue of astragalus (10, 20 g/kg) pretreatment groups were significantly decreased, and the level of IL-1β in 20 g/kg pretreatment group was significantly decreased and the IL-10 was significantly increased. The mesentery cells apoptosis in astragalus pretreatment groups were significantly improved and the AI in astragalus(10, 20 g/kg) pretreatment groups was significantly decreased and the activity of Caspase-3, Caspase-9 in mesentery tissue were significantly decreased, and the expression of NF-κB was significantly decreased;all of the difference above were significant (P<0.05 or P<0.01). [Conclusion] Astragalus had inhibitive effects on mesentery in ischemia-reperfusion rats, which perhaps related to its effects of enhancing the activity of depressing oxidative stress, inhibiting inflammation and apoptosis.关键词
黄芪预处理/肠系膜/缺血再灌注/影响/机制Key words
astragalus pretreatment/mesentery/ischemia-reperfusion/effect/mechanism分类
医药卫生
