中国药理学与毒理学杂志2016，Vol.30Issue(9)：941-948,8.DOI:10.3867/j.issn.1000-3002.2016.09.006
氯吡硫磷染毒撤除对原代培养大鼠海马神经元细胞毒性的延迟效应
Chlorpyrifos exposure withdrawal induces delayed cytotoxicity in rat primary hippocampal neurons
摘要
Abstract
OBJECTIVE To investigate the delayed cytotoxicity effect of chlorpyrifos (CPF) with⁃drawal on primary hippocampal neurons. METHODS Hippocampal neurons were prepared from SD rat fetuses on the 17th day of gestation. Seven days after culture,neurons were exposed to CPF 10 and 30 μmol · L-1,respectively,for 72 h or for 48 h followed by CPF withdrawal for 24 h. CCK-8 kit and neuronal nuclei(NeuN), 5-bromodeoxyuridine(BrdU) and β Ⅲ tubulin immunofluorescence expression methods were used to evaluate the cell viability. RESULTS Compared with normal control, no significant cytotoxicity was found after CPF 72 h continuous exposure. However,CPF 48 h expo⁃sure followed by CPF withdrawal for 24 h induced evident cytotoxicity. The amount of BrdU positive and β Ⅲ tubulin positive hippocampal neurons were both decreased significantly(P<0.05),and cell survival and viability reduced after CPF withdrawal. CONCLUSION CPF exposure withdrawal can induce more seriously delayed cytotoxicity than continuous exposure in rat primary hippocampal neurons.关键词
有机磷杀虫剂/氯吡硫磷/海马/神经元,培养的Key words
organophosphorus insecticides/chlorpyrifos/hippocampus/neurons,cultured分类
医药卫生引用本文复制引用
吴春燕,闫长会,傅风华,关勇彪..氯吡硫磷染毒撤除对原代培养大鼠海马神经元细胞毒性的延迟效应[J].中国药理学与毒理学杂志,2016,30(9):941-948,8.基金项目
国家自然科学基金（81373035）@@@@The project supported by National Natural Science Foundation of China （）
