中国药业2016，Vol.25Issue(17)：35-37,3.
丁酸盐二甲双胍对乳腺癌干细胞的抗肿瘤活性及细胞毒性作用研究
The Enhancement of Anti-Tumor Activity and Cytotoxicity on Breast Cancer Stem Cells by Metformin Hydrochloride
王文蓉1
作者信息
- 1. 湖北省荆州市第三人民医院，湖北 荆州 434000
- 折叠
摘要
Abstract
Objective To study the enhancement of anti-tumor activity and cytotoxicity of metformin hydrochloride on breast cancer stem cells. Methods The breast cancer stem cells were obtained by ultra-low adherence suspension culture from MDA-MB-231 cells. The breast cancer stem cells were randomized into the control group ( doxorubicin ) and the observation group ( metformin hy-drochloride combined with doxorubicin ) . The cells were controlled under hypoxia. The cell apoptosis in two groups was detected by flow cytometry. The proliferation of breast cancer stem cells was assayed by MTT measurement. Results In the control group, the treatment of metformin hydrochloride could promote the cell apoptosis of MDA-MB-231 without obvious effect on ER-positive MCF-7; in the observation group, the treatment of metformin hydrochloride could significantly increase the sensitivity of breast cancer stem cells to chemotherapy drug. Conclusion Metformin hydrochloride can promote the apoptosis of breast cancer stem cells and increase the sensi-tivity of breast cancer stem cells to chemotherapy drug.关键词
丁酸盐二甲双胍/乳腺癌干细胞/细胞凋亡/细胞增殖Key words
metformin hydrochloride/breast cancer stem cells/cell apoptosis/cell proliferation分类
医药卫生引用本文复制引用
王文蓉..丁酸盐二甲双胍对乳腺癌干细胞的抗肿瘤活性及细胞毒性作用研究[J].中国药业,2016,25(17):35-37,3.
