中国药业2016，Vol.25Issue(18)：21-24,4.
Ⅱ型大麻受体激动剂JWH133对大鼠局灶性脑缺血－再灌注损伤的神经保护作用
Protective Effect of CB2 Agonist JWH133 on Cerebral Ischemia-Reperfusion Injury in Rats
摘要
Abstract
Objective To investigate the effect of CB2 agonist JWH133 pretreatment on focal cerebral ischemia / reperfusion injury in rats and its underlying mechanism. Methods 40 healthy adult male SD rats were randomly divided into 4 groups( n=10):sham opera-tion group,model group,JWH133 group and solvent control group. The modified ZeaLonga suture method was used to build the models with,theLonga score was used to evaluate the neurological function,the TTC staining methodwas used to determine the content of is-chemic brain tissue and peripheral serum IL-6 and IL-1β,and the TUNEL immunofluorescence stain method was used to detect the apoptosis of rat neurons. Results The rats in the four groups all had different degrees of abnormal neurological function. The JWH133 group was significantly better in the neurological recovery than the other 3 groups( P < 0. 05). Brain slice staining showed that all groups had white perforating artery infarct,but the infarctin the JWH133 group was smaller than the model group and control group ( P < 0. 05). The levels of IL-6 and IL-1β in serum and brain tissue of rats in the model group were significantly higher than the sham operation group( P < 0. 05),and the two inflammatory factors were reduced after treatment of JWH133( P < 0. 05). TUNEL stain-ing showed that there were almost no apoptotic cells in the sham operation group,the number of model group and solvent control group apoptotic cells was significantly increased,and the apoptotic neurons in JWH133 intervention group were significantly de-creased. Conclusion JWH133 preconditioning may play a neuroprotective role in cerebral ischemia-reperfusion injury,and its mecha-nism may be related to reducing the levels of IL-6,IL-1β,and playing the role of antiapoptosis. The mechanism of the neuroprotec-tive effect of CB2 agonist JWH133 remains to be further explored.关键词
Ⅱ型大麻受体激动剂/JWH133/脑缺血-再灌注/神经保护作用Key words
CB2 agonist/JWH133/cerebral ischemia-reperfusion/neuroprotective effect分类
医药卫生引用本文复制引用
唐中华,王强,封海涛,李晶..Ⅱ型大麻受体激动剂JWH133对大鼠局灶性脑缺血－再灌注损伤的神经保护作用[J].中国药业,2016,25(18):21-24,4.基金项目
重庆市基础与前沿研究计划项目，项目编号cstc2014jcyjA10049；第二批重庆市高等学校青年骨干教师资助计划项目（2014）；重庆市社会民生科技创新专项，项目编号cstc2016shmszx130028。 （）
