中国药业2016，Vol.25Issue(18)：25-28,29,5.
超高效液相色谱－质谱联用法测定藏药萨热十三味鹏鸟丸中乌头碱类含量
Content Determination of Aconitine,Hypaoconitine and Mesaconitine in Sare Shisanwei Pengniao Pills by UPLC-MS
摘要
Abstract
Objective To establish an UPLC-MS method for the content determination of aconitine,hypaoconitine and mesaconitine in SareShisanweiPengniao Pills. Methods The microscopic identification method was used to identifythe aconitum in agents;the chro-matographic column was AcQuity UPLC BEH C18 column,mobile phase A was methanol and mobile phase B was 0. 1% formic acid, gradient elution;the electrospray ionization(ESI),positive ion scan(ES+) and multiple reaction monitoring(MRM) were used todeter-minethe contents of aconitine,hypaoconitine and mesaconitine in SareShisanweiPengniao Pills. Results The linear relationship was good in the range of 13. 15-2 630. 00 ng for aconitine( r=0. 999 4),and the average recovery rate was 97. 34%,RSD=1. 79%( n=9);the linear relationship was good in the range of 32. 06-3 205. 89 ng for hypaoconitine( r=0. 999 9),and the average recovery rate was 96. 09%,RSD=1. 94%( n=9);the linear relationship was good in the range of 10. 23-2 046. 00 ng for mesaconitine( r=0. 999 3), and the average recovery rate was 95. 94%,RSD=1. 75%( n=9). Conclusion This microscopic identification method is simple and sensitive with significantly clear characteristics and easy to identify results;the UPLC-MS method is accurate and reliable with good repeatability,and can be used for the content determination aconitine,hypaoconitine and mesaconitine in SareShisanweiPengniao Pills.关键词
萨热十三味鹏鸟丸/显微鉴别/超高效液相色谱-质谱联用法/乌头碱/次乌头碱/新乌头碱Key words
SareShisanweiPengniao Pills/microscopic identification/UPLC-MS/aconitine/hypaoconitine/mesaconitine分类
医药卫生引用本文复制引用
魏文芝,海平,骆桂法..超高效液相色谱－质谱联用法测定藏药萨热十三味鹏鸟丸中乌头碱类含量[J].中国药业,2016,25(18):25-28,29,5.基金项目
青海省科技厅资助项目，项目编号2012－N－523。 （）
