安徽农业科学2016，Vol.44Issue(24)：123-124,154,3.
猴头菌子实体与菌丝体粗多糖理化性质及抗胃粘膜细胞氧化活性比较
Comparison of Physicochemical Propetr ies between Hericium Erinaceus Mycelium and Fruit Body Crude Polysaccharides and Their Anti-oxid ation Effects of Gastric Mucosal Cel
摘要
Abstract
Objective] To compare the physicochemical properties of crude polysaccharides compare of Hercium erinaceus mycelium and fruit body, and to compare the differences of the effects of two crude extracts on oxidation protection ability of gastric mucosa epithelial cell.[ Meth-od] Crude crude polysaccharides were extracted by water-extraction and alcohol-precipitation method.Glucose content was detected by phenol-sulphuric acid method; uronic acid content was detected by m-hydroxydiphenyl method; and protein content was detected by BCA method. Culture medium containing certain content of polysaccharides compare was added into each group.After cultivated for 12, 24 and 48 h, cul-ture medium containing hydrogen peroxide was added.Viability of cells was detected by MTT.[ Result] Glucose contents in mycelium and fruit body were 49.5% and 44.5%, respectively.The uronic acid contents were 22.01% and 29.23%; protein contents were 27.69% and 23.54%.The inhibitory rates of mycelium group at 12, 24 and 48 h were 75.1%, 68.1% and16 .9%, respectively;those of fruit body were 70.2%, 61.8% and 16.7%, respectively.[Conclusion]In the crude crude polysaccharides of mycelium and fruit body of Hecr ium erina-ceus, contents of uronic acid, glucose and protein are close.Crude polysaccharides of fruit body have stronger antioxidant activity than extracts of mycelium in short term, but there were no differences in long term.关键词
猴头菌菌丝体/猴头菌子实体/理化性质/胃粘膜细胞/抗氧化Key words
Heric um Erinaceus mycelium/Hercium erinaceus fruit body/Physicochemical properties/Gastric mucosal cell/Antioxidant分类
农业科技引用本文复制引用
肖旭朗,薛德冬,张薇,张洪贺,高阳,徐多多,王明星..猴头菌子实体与菌丝体粗多糖理化性质及抗胃粘膜细胞氧化活性比较[J].安徽农业科学,2016,44(24):123-124,154,3.基金项目
国家自然科学基金项目（30873370）。 （）
