安徽农业科学2016，Vol.44Issue(24)：127-130,4.
草豆蔻有效成分的半制备型高效液相色谱分离
Separation of Active Components in Alpinia katsumadai Hayata by Semi-preparative High Performance Liquid Chromatography
摘要
Abstract
Objective] The aim was to develop a semi-preparative high performance liquid chromatography (SP-HPLC) method for separation and purification of four effective components from Alpinia katsumadai Hayata.[Method] Using C18 column (250 mm ×25.4 mm I.D.,10 μm), the optimum separation conditions were obtained through investigating the effects caused by the composition and flow rate of the mobile phase and the sample size.Elution was performed by preparative chromatography,product effluent was collected online according to detection signal,the pu-rity of separated fraction was detected by HPLC method.[ Result] The suitable conditions were listed as follows:the mobile phase was methanol-water in stepwise elution mode,the flow rate of the mobile phase was 25 mL/min,the sample size was 232 mg,and the detection wavelength was 300 nm.Under the above conditions,four compounds obtained purification through one-step separation.They were identified as alpinetin,pino-cembrin,cardamonin and alnustone by ultraviolet spectrum (UV) and nuclear magnetic resonance (NMR) analysis,and the purities of the four compounds were 98.7%,99.3%,98.4%and 99.1%as determined by high performance liquid chromatography (HPLC).[Conclusion] Compa-ring with the traditional separation method,the SP-HPLC method developed in the paper has many advantages such as high efficiency,facility,and high speed.And it was suitable for rapid preparation of effective components from A.katsumadai.关键词
半制备型高效液相色谱/草豆蔻/有效成分/分离Key words
Semi-preparative high performance liquid chromatography/Alpinia katsumadai Hayata/Active ingredient/Separation分类
农业科技引用本文复制引用
王延翠,李爱峰,孙爱玲,柳仁民..草豆蔻有效成分的半制备型高效液相色谱分离[J].安徽农业科学,2016,44(24):127-130,4.基金项目
山东省自然科学基金（ZR2014BQ023）。 （）
