安徽农业科学2016，Vol.44Issue(24)：155-156,172,3.
一种野山参微量DNA的提取方法
A Kind of Wild Ginseng Trace DNA Extratc ion Method
孙薛蛟 1骆红梅2
作者信息
- 1. 贵阳中医学院，贵州贵阳550002
- 2. 贵阳中医学院第二附属医院，贵州贵阳550001
- 折叠
摘要
Abstract
Objective] The aim was to optimize CTAB method for DNA extraction from a small amount of fibrous roots of wild ginseng to keep the integrity form of wild ginseng.[Method] CTAB extraction method and other three kinds of DNA extraction method were comprehensively compared, CTAB method which is suitable for DNA extraction from a small amount of medicinal herbs was selected.[Result] DNA was extrac-ted from 5 mg ginseng root, the concentration of cultivated ginseng DNA was 200-1 000 ng/μL, wild ginseng was 50-100 ng/μL, electropho-resis can detect clear strip, meet the requirements of the concentration and purity.[ Conclusion] The improved high salt SDS method can extract high quality DNA from 5 mg trace ginseng fibril material.This method meet the requirements of the concentration and purity, and can be the ref-erence method for extracting DNA from other medicinal materials.关键词
栽培参/野山参/DNA提取Key words
Cultivated ginseng/Wild ginseng/DNA extraction分类
农业科技引用本文复制引用
孙薛蛟,骆红梅..一种野山参微量DNA的提取方法[J].安徽农业科学,2016,44(24):155-156,172,3.
