安徽农业科学2016，Vol.44Issue(24)：160-162,3.
西番莲离体快繁研究
Research on Rapid Propagation in vitro of Passiflora caerulea L.
董丽兰 1岑秀芬 2韦鹏霄2
作者信息
- 1. 广西贺州市平桂管理区水果生产办公室，广西贺州542827
- 2. 广西大学农学院，广西南宁530005
- 折叠
摘要
Abstract
Objective] The aim was to build a fast and efficient regeneration system of Passiflora caerulea L.in vitro.[Method] One year stems, leaves and petioles of P.caerulea were used as propagation materials in vitro, and studies focused on the selection of induced tissues, different time of getting materials, callus induction and differentiation, reproduction of irregular buds and induction of taking roots were con-ducted in detail.[Result] The results showed that MS+6-BA 1.0 mg/L+NAA 0.2 mg/L +TDZ 0.05 mg/L was comparatively superior, in-duction rate of stem tips of which is 96.6%, petioles is 88.4%, and leaf is 83.5%; MS+6-BA 1.0 mg/L +NAA 0.5 mg/L +TDZ 0.05 mg/L showed optimal, and the rate of differentiated multiple shoots of which is 92.8%; the induction rate involved with adventitious buds of MS+6-BA 2 mg/L +NAA 0.2 mg/L +TDZ 0.05 mg/L is 90.9%; the propagation times of buds of MS +6-BA 2.5 mg/L +NAA 0.2 mg/L is 2.91; the number of average taking roots of 1/2MS+NAA 2 mg/L +PP333 0.5 mg/L is 1.3; the rate of taking roots of 1/2MS+NAA 2 mg/L +ABT 0.5 mg/L is 95.4%;the average root length of 1/2MS+NAA 2 mg/L +IBA 0.5 mg/L is 1.23 centimeter.[Con-clusion] The study lays a foundation for breeding and integrated utilization of P.caerulea in future.关键词
西番莲/组织培养/快速繁殖Key words
Passiflora caerulea L./Tissue culture/Rapid propagation分类
农业科技引用本文复制引用
董丽兰,岑秀芬,韦鹏霄..西番莲离体快繁研究[J].安徽农业科学,2016,44(24):160-162,3.
