北京林业大学学报2016，Vol.38Issue(8)：18-27,10.DOI:10.13332/j.1000-1522.20160041
毛果杨基因PtNRT2.7的功能初步鉴定与分析
Functional identification and analysis of PtNRT2 7 gene from Populus trichocarpa
摘要
Abstract
In present study, we successfully isolated a gene from NRT2 family named PtNRT2. 7. Bioinformatic analyses showed that PtNRT2. 7 had 11 transmembrane regions, and hydrophobic regions and hydrophilic regions are distributed alternately on cell membrane without signal peptide. Subcellular localization analysis showed that the PtNRT2. 7-GFP fusion protein is distributed on cell membrane. According to above findings, it belongs to a membrane protein. The expression features of Populus trichocarpa showed that PtNRT2. 7 expresses in leaves, with the highest expression in mature leaves; the highest expression appears at 1 h under the nitrate stress;under the stress of SA/JA/GA/IAA, the expression of PtNRT2. 7 is up-regulated in both above-and underground parts;under the stress of Eth/ABA/NaCl, the expression of PtNRT2. 7 is down-regulated. The expression vector was constructed and transformed into Arabidopsis thaliana wild type ( Col-0 ) and atnrt2. 7 mutant using floral dip method. The stress of different concentrations of KNO3 showed that, when the concentration of KNO3 is more than 0. 1 mmol/L, the root enlongation of overexpression lines is longer than others; the mutant lines are inhibited when the concentration is more than 1 mmol/L but the complementary lines can remove the inhibition. The result showed that hormones and NO3- can induce PtNRT2. 7, enhance the root growth and promote the absorption of nitrogen.关键词
毛果杨/PtNRT2.7基因/序列分析/功能鉴定Key words
Populus trichocarpa/PtNRT2. 7/sequence analysis/functional identification分类
农业科技引用本文复制引用
唐贤礼,张月,张盾,夏新莉,尹伟伦..毛果杨基因PtNRT2.7的功能初步鉴定与分析[J].北京林业大学学报,2016,38(8):18-27,10.基金项目
林业公益性行业科研专项(201304301)、国家自然科学基金项目(31270656)和高等学校学科创新引智计划项目(111 Project、B13007)。 （201304301）
