北京林业大学学报2016，Vol.38Issue(8)：80-87,8.DOI:10.13332/j.1000-1522.20150377
紫斑牡丹表型性状与SSR分子标记的关联分析
Association analysis of phenotypic traits with SSR markers in Paeonia rockii
摘要
Abstract
In order to provide a genetic basis for studies on complex quantitative traits and for molecular marker assisted breeding of tree peony, the genetic diversity of Paeonia rockii was determined by using SSR markers and their association with important ornamental traits was detected. A total of 11 simple sequence repeat ( SSR ) markers were screened for polymorphism among 99 accessions, and then the genetic diversity of these materials was detected. Through analyzing population structure, association analysis between SSR markers and 32 horticultural traits were performed using TASSEL2. 1 GLM ( general linear model) programs. The results showed that a total of 94 alleles were identified with 8. 5 alleles per locus. The polymorphism information content ( PIC ) value ranged from 0. 146 to 0. 850 , with an average of 0. 593 . The genetic diversity ranged from 0. 152 to 0. 862 , with the mean of 0. 630 . Population structure analysis showed that 99 Paeonia rockii materials were composed of 3 subpopulations. Association analysis found that there were 5 SSR markers associated with 6 horticultural traits, and the rate of explanation on the phenotype of related marker ranged from 30. 4% to 55. 8%. Among them, FJ024285 and FJ024294 were associated with plant height; FE528073 was associated with top-leaf length, stigma color and disk color;FJ024287 was associated with length ratio of petiole and compound leaf;EU678295 was associated with blotch size. An abundant genetic variation but week population structure was detected in Paeonia rockii accessions, indicating that the association population is representative and suitable for further association analysis of quantitative traits in tree peony. Association analysis is a promising approach to find markers tightly linked with phenotypic traits, and the markers obtained in the present study provide foundation for molecular marker assisted breeding.关键词
紫斑牡丹/SSR标记/遗传多样性/群体结构/关联分析Key words
Paeonia rockii/SSR marker/genetic diversity/population structure/association analysis分类
农业科技引用本文复制引用
吴静,成仿云,庞利铮,钟原,蔡长福..紫斑牡丹表型性状与SSR分子标记的关联分析[J].北京林业大学学报,2016,38(8):80-87,8.基金项目
“863”国家高技术研究发展计划项目(2011AA100207)。 （2011AA100207）
