吉林大学学报（医学版）2016，Vol.42Issue(5)：855-859,5.DOI:10.13481/j.1671-587x.20160503
多效生长因子对小鼠子宫基质细胞蜕膜化的影响
Effect of pleiotrophin on decidualization of uterine stromal cells in mice
摘要
Abstract
Objective:To construct the pleiotrophin (PTN )overexpression vector,and to explore the effect of PTN on the decidualization of uterine stromal cells in the mice.Methods:The specific primers containing restriction enzyme cutting sites were designed according to the PTN gene sequences published in GenBank for PCR amplification.The amplified fragment of PTN was recovered from the agarose gel and cloned into the pGEM-T vector.The pGEMT-PTN was cut by double enzyme digestion and ligated into pcDNA3.1 (+)to construct the PTN overexpression plasmid.After transfection with PTN overexpression plasmid,the expression levels of PTN mRNA in the uterine stromal cells and the expression levels of decidualization markers Prl8a2 and Prl3c1 were detected by qRT-PCR method.The uterine stromal cells transfected with pcDNA3.1 (+)empty vector were used as control group. Results:The results of identification by double enzyme digestion indicated that the bands of PTN overexpression plasmid were consistent with those of the target gene,and the clone sequencing results suggested that it had 100% homology with mouse PTN gene sequence published in GenBank.Compared with control group, the expression levels of PTN,Prl8a2 and Prl3c1 mRNA in mouse uterine stromal cells in PTN overexpression group were significantly increased (P < 0.05).Conclusion:PTN overexpression could increase the expression levels of decidualization markers in mouse uterine stromal cells,indicating that PTN might play an enhancement effect during uterine decidualization in the mice.关键词
多效生长因子/载体构建/转染/蜕膜化/小鼠Key words
pleiotrophin/vector construction/transfection/decidualization/mice分类
医药卫生引用本文复制引用
于海帆,郭传辉,李当当,张虹亮,耿爽,杨占清,郭斌,岳占碰..多效生长因子对小鼠子宫基质细胞蜕膜化的影响[J].吉林大学学报（医学版）,2016,42(5):855-859,5.基金项目
国家自然科学基金资助课题 （）
