吉林大学学报（医学版）2016，Vol.42Issue(5)：932-936,5.DOI:10.13481/j.1671-587x.20160518
Disabled-1在人乳腺癌细胞中的表达及其对细胞周期的影响
Expression of Disabled-1 in human breast cancer cells and its role in cell cycle
摘要
Abstract
Objective:To explore the expressions of Disabled-1 (Dab1 )in human breast epithelial cells and breast cancer cells,and to clarify its role in cell cycle.Methods:Real-time PCR was used to analyze the Dab1 mRNA expressions in breast epithelial cells MCF-10A and breast cancer cells MCF-7,BT-549,and MDA-MB-231. The Dab1 protein expressions in those cells were tested by Western blotting method. The BT-549 cells at logarithmic growth phase were divided into control,pKH3,and pKH3-Dab1 groups;the cell cycle was investigated by flow cytometry.Results:The Real-time PCR results showed that the Dab1 mRNA expression levels in MCF-7 cells (0.504 ± 0.037),BT-549 cells (0.302 ± 0.027),and MDA-MB-231 cells (0.330 ± 0.031 )were reduced compared with MCF-10A cells (0.998±0.020)(P <0.05).The Western blotting results showed that the Dab1 protein expression levels in breast cancer cells MCF-7 (0.134±0.014),BT-549 (0.076±0.01),and MDA-MB-231 (0.074±0.005)were reduced compared with MCF-10A cells (0.227±0.021)(P <0.05).Compared with control group and pKH3 group,the cell cycle in pKH3-Dab1 group was inhibited at G1 phase detected by flow cytometry analysis. Conclusion:The expression of Dab1 is down-regulated in breast cancer cells,and the over-expression of Dab1 can inhibit the cell cycle at G1 phase.关键词
Dab1基因/人乳腺癌细胞/细胞周期/G1期阻滞Key words
Dab1/human breast cancer cell/cell cycle/G1 phase inhibition分类
医药卫生引用本文复制引用
曹让娟,李凯,邢琬莹,王悦书,于维,吴广智,崔树森,李强..Disabled-1在人乳腺癌细胞中的表达及其对细胞周期的影响[J].吉林大学学报（医学版）,2016,42(5):932-936,5.基金项目
国家自然科学基金资助课题（81502516）；吉林省科技厅科技发展计划项目资助课题（20160101077JC）；吉林省科技厅医药产业发展引导资金项目资助课题（20150311069YY）；吉林大学白求恩计划项目青年基金资助课题 （）
