草业学报2016，Vol.25Issue(9)：37-45,9.DOI:10.11686/cyxb2016104
三种作物茎叶枯落物水浸液对烟草幼苗生长的化感效应
Allelopathic effects of leaf-stem litter water aqueous extracts of three plant species on tobacco seedlings
摘要
Abstract
Tobacco,and the two Chinese herbal medicine crops Scrophularia ningpoensis and Chuanminshen violaceum ,all present husbandry difficulties under continuous cropping systems,and allelopathic effects are considered to be a significant contributing factor.There have been relatively few studies of auto-allelopathy in tobacco crops or of allelopathy between tobacco and the Chinese herbal medicine crops S .ningpoensis and C. violaceum.To investigate problems arising in continuous cropping of tobacco,a study was conducted on leachate effects on seedlings.Flue-cured tobacco K126 and 1 year old leaf and stem litter were used to prepare aqueous extracts of leachates of tobacco (LeT),and leaf and stem litter were also used to prepare aqueous ex-tracts of S .ningpoensis (LeS),and C.violaceum (LeC),with pure water as control.Treatment concentra-tions were 0.01,0.02,0.05,0.10 mg/L.LeT had an inhibitory effect on seedlings below ground and above ground growth,as indicated by elevated antioxidant enzyme activity.For LeS the highest concentration of 0.10 mg/L showed significant inhibition,while the lowest concentration of 0.01 mg/L had a stimulatory effect. Meanwhile,LeC had little impact on tobacco seedlings,or tended to induce positive growth promotion effects. Following application of the aqueous extracts of S .ningpoensis and C.violaceum,SOD,POD,CAT activities were increased with increasing concentration of the extracts,and soluble protein,initial chlorophyll fluores-cence (F 0 ),the maximum enhanced fluorescence (F m ),variable fluorescence (F v ),PSⅡ potential photochem-ical activity (F v/F 0 ),and PSⅡ maximum photochemical efficiency (F v/F m ),also showed better adaptation. Therefore,S .ningpoensis and C.violaceum may be an alternative rotation or intercropping plant species for tobacco,and intercropping may help ease husbandry problems encountered in continuous cropping of tobacco.关键词
烟草/玄参/川明参/连作障碍/化感作用Key words
tobacco/Scrophularia ningpoensis/Chuanminshen violaceum/continuous cropping obstacles/al-lelopathic effects引用本文复制引用
赵新梅,王军,莫静静,杨水平,温明霞,张雪,赵建,陈大霞,蒋卫..三种作物茎叶枯落物水浸液对烟草幼苗生长的化感效应[J].草业学报,2016,25(9):37-45,9.基金项目
贵州省烟草公司遵义市公司研究开发项目（201404），中央高校基本科研业务费专项资金（SWU113094），国家自然科学基金（31370602），重庆市科委“科技平台与基地建设”项目（CSTC，2014ptyjd10001）和重庆市卫生和计生委中医药科技项目（ZY201402132）资助。 （）
