广西植物2016，Vol.36Issue(9)：1032-1038,7.DOI:10.11931/guihaia.gxzw201504028
21种野生百合亲缘关系的ISSR分析
Genetic relationship analysis of 21 wild species of Lilium by ISSR markers
摘要
Abstract
To probe the infrageneric phylogenetic relationship of Lilium, seven inter-simple sequence repeat ( ISSR) primers were chosen to analyze genetic diversity of 21 wild species of Lilium. POPGENE1.32 and MEGA5.1 were used for data analysis. Results showed that a total of 149 clear DNA bands were amplified, 136 of which were polymorphic, the proportion was 91.06%. The average value of effective number of alleles was 1.762 4. The average value of Nei’ s gene diversity was 0.421 4, and the average value of Shannon's Information index was 0.608 5. The genetic distance ranged from 0.307 5 to 0.887 3. The genetic distance between L. leucanthum and L. leucanthum var. centifolium was a maximum of selected 21 parts of wild lily material, average value 0.887 3, which indicated that the genetic relationship between them was the fartherest. Whereas the genetic distance between L. leucanthum var. centifolium and L. lophopho-rum was a minimum of selected 21 parts of wild lily material, average value 0.307 5, which indicated genetic relation-ship between them was the closest. The clustering results were basically consistent with the morphology classification. Twenty-one wild Lilium species were classified into five clustering groups by neighbor joining method. L. taliense, L. duchartrei, L. lancifolium, L. nepalense, L. papilliferum, L. davidii, L. davidii var. unicolor, L. pumilum, L. fargesii and L. dauricum were clustered in the first group. And L. leucanthum Baker var. centifolium, L. leucanthum, L. nepalense, L. regale, L. sargentiae belong to the second group. L. brownie, L. brownii var. viridulum were clustered in the third group. L. tsingtauense formed a distinct group and L. lophophorum, L. amoenum, L. concolor var. pulchellum were clustered in the fifth group. L. dauricum has close genetic relationship with sect. Sinomartagon, and there were gene exchanges existed between Sect. Lophophorum and Sect. Sinomartagon. This indicated that it was not accurate to classify the wild Lilium all depend on the morphology. L. brownii var. viridulum and L. brownie were clustered into one individual group in cluster analysis results. This result showed that whether it has petioles was an im-portant morphological character in the classification of wild lilies. And all the results indicated that ISSR markers were suited to study the phylogenetic relationship of wild lilies.关键词
百合属/野生百合/ISSR/亲缘关系/树状图Key words
Lilium/wild lilies/ISSR/genetic relationship/tree plot分类
农业科技引用本文复制引用
侯珺,罗建让,肖菲菲,张延龙,牛立新..21种野生百合亲缘关系的ISSR分析[J].广西植物,2016,36(9):1032-1038,7.基金项目
国家林业局重点科研项目(2006-73)；陕西省林业厅项目(陕林计字[2011]70号)；国家自然科学基金(305021110)；西北农林科技大学基本科研业务费专项(Z109021002)[Supported by Key Scientific Research Project of State Forestry Administration (2006-73)； Project of Shaanxi Forestry Department ([2011]70)；National Natural Science Foundation of China (305021110)；Foundamental Scientific Research Fund of Northwest A&F University ( Z109021002)]。 （2006-73）
