广西植物2016，Vol.36Issue(9)：1052-1060,9.DOI:10.11931/guihaia.gxzw201503026
鹅掌楸DHHC型锌指蛋白家族基因的克隆及表达分析
Gene cloning and expression analysis of DHHC protein family genes from Liriodendron chinense
摘要
Abstract
Protein S-acylation is a common and unique reversible way in posttranslational lipid modification, thus confers diverse physiological functions on target proteins, such as DHHC ( Asp-His-His-Cys) protein family. There was evidence that the DHHC domain was directly involved in the palmitoyl transfer reaction. In this paper, we reports gene cloning and expression analysis of DHHC protein family genes in Liriodendron chinense. Three full-length cDNA of DH-HC gene were cloned from Liriodendron leaf buds using the rapid amplification of cDNA ends ( RACE) strategy, named LcPAT7, LcPAT22 and LcPAT23 respectively. The full-length cDNAs of LcPAT7, LcPAT22 and LcPAT23 were 1 933 bp, 2 592 bp and 2 217 bp, and contain 1 332 bp, 1 839 bp and 1 662 bp ORF, encoding 433, 612 and 533 amino acids, respectively. The predicted molecular weights of the proteins encoded by LcPAT7, LcPAT22 and LcPAT23 were 40.04,67.3 and 60.57 kDa, respectively. The predicted isoelectric points are 9.15, 9.03 and 7.29, respectively. All the three proteins contained four putative transmembrane ( TM) domain structures, and contained a typically DHHC-CRD domain between TM2 and TM3 as most known DHHC protein. Homology analysis showed that the three genes showed high simi-larity with predicted PAT (protein S-acyltransferase) of other plants. Tissue expression profile by Real-time PCR showed that all the three genes were expressed in various tissues, although the expression levels varied significantly in different tissues. Changes in gene expression patterns in the same gene family indicated their functionally non-redundancy. These results above will provide clues for exploring the underlying mechanism of gene regulation on growth, development, mor-phogenesis and signal transduction of stress response in L. chinense.关键词
鹅掌楸/棕榈酰化修饰/蛋白质酰基转移酶/DHHC蛋白家族/表达分析Key words
Liriodendron chinense/protein S-acylation/protein S-acyltransferase (PAT)/DHHC protein family/ex-pression analysis分类
农业科技引用本文复制引用
徐嘉娟,李火根..鹅掌楸DHHC型锌指蛋白家族基因的克隆及表达分析[J].广西植物,2016,36(9):1052-1060,9.基金项目
国家自然科学基金(31470660,31170621)；江苏省高校优势学科[ Supported by the National Natural Science Foundation of China (31470660,31170621)；Priority Academic Program Development of Jiangsu High Education Institutions]。 （31470660,31170621）
