广西植物2016，Vol.36Issue(9)：1112-1118,7.DOI:10.11931/guihaia.gxzw201509025
蛇足石杉中的新内生真菌及其代谢产物的分离与鉴定
Isolation and identification of secondary metabolites from a new endophyte Huperzia serrata
摘要
Abstract
Many kinds of endophytic fungi are widely associated with various plants and a lot of systemic researches focu-sing on secondary metabolites of endophytes had been conducted. However, the research on endophyte in Huperzia serra-ta is rare, which is a traditional Chinese medicinal fern Qian Ceng Ta, belongs to Huperzia of Huperziaceae. Finding ac-tive compounds from endophytes in H. serrata can provide a new way for further exploitation resources of this medicinal plant. The purpose of this study was to search cytotoxic active compounds of endophytes from H. serrata. We isolated the chemical constitution of Chaetomium sp. M336 which was an endophytic fungus isolated in the roots of Huperzia serrata. The Chaetomium sp. M336 was fermented on solid PDA media. The fungus containing with medium was extracted with ethyl acetate ∶ methanol ∶ acetic acid = (80∶ 15∶ 5, V/V/V) mixed organic solvent, and the secondary metabo-lites of endophytic fungi were obtained. The compounds of the fermented extracts were separated and purified by column chromatography involving normal-phase silica gel, Sephadex LH-20 and reversed-phase semi-preparative HPLC. Their structure of the compounds were elucidated by their physical and chemical properties, including nuclear magnetic reso-nance ( NMR) spectroscopy, and electrospray ionization mass spectrometry ( ESI-MS) and according to the reported lit-eratures. They results were as follows:eight compounds were isolated and purified from the ethyl acetate extracts of the fungus Chaetomium sp. M336. They were identified as chaetoviridines F, chaetoviridines E, 5′-epichaetoviridin A, 5′-epichaetoviridin A, xanthoquinodins Al, xanthoquinodins A2, xanthoquinodins B1, chetomin. In conclusion, eight com-pounds were isolated from M336. Compound 3 showed some certain anti-bacterial activity, others exhibit cytotoxicity. This preliminary work will provide information for further search on natural cytotoxic activity products of en-dophytic fungi from Huperzia serrata.关键词
球毛壳属/内生真菌/结构鉴定/细胞毒活性Key words
Chaetomium sp./endophytic fungi/structure identification/cytotoxicity分类
农业科技引用本文复制引用
于飞雪,杨银河,赵沛基,陈瑶..蛇足石杉中的新内生真菌及其代谢产物的分离与鉴定[J].广西植物,2016,36(9):1112-1118,7.基金项目
国家重点基础研究发展“973”计划项目(2013CB127505)[Supported by the National Basic Research Program of China “973” Program,(2013CB127505)]。 （2013CB127505）
