广西植物2016，Vol.36Issue(9)：1119-1125,7.DOI:10.11931/guihaia.gxzw201502004
响应面法优化干花豆总黄酮提取工艺研究
Optimization of extracting total flavonoids from Fordia cauliflora by response surface methodology
摘要
Abstract
Fordia cauliflora is the genus Fordia in the family Leguminosae, which contains flavonoids, alkaloids, organ-ic acids and so on, possesses various activities such as beneficial wisdom, anti-aging, anti-inflammatory. The current re-search of F. cauliflora mainly focuses on the study of chemical constituents and pharmacological activities, there is little report about the optimization of extraction process of flavonoids. In order to determine the best extraction technology of to-tal flavonoids from F. cauliflora and evaluate their antioxidant activity, the extraction temperature, solid-liquid ratio, ethanol concentration and extraction time as single factor were tested and then the extraction process of the total fla-vonoids from F. cauliflora was optimized by response surface designs based on single factor experiments. In addition, the antioxidant activity of flavonoids from F. cauliflora was measured by using 1, 1-diphenyl-2-picryhydrazyl radical (DPPH.) and hydroxyl radical (.OH) scavenging. The results showed that total flavonoids of optimum extraction conditions of F. cauliflora were as follows:extraction temperature 78℃, solid-to-liquid ratio of 1∶ 30( g.mL-1 ) , the alcohol concentration for 71%, extraction time 187 min. Under these conditions, an extraction rate of 10.53 mg.g-1 for total flavonoids from F. cauliflora was obtained, compared with the predictive value of 10.61 mg.g-1 , the relative error was 0.76% . The IC50 values of scavenging DPPH and OH radical were 14.09 and 78.43μg.mL-1, weaker than that of Vc(8.11 and 67.95μg.mL-1). The extraction process was stable and reasonable, accurate and reliable, and it can be used to extract the total flavonoids from F. cauliflora. The results will provide basis for the further development and utili-zation of flavonoids of F. cauliflora.关键词
干花豆/总黄酮/响应面法/抗氧化性Key words
Fordia cauliflora/flavone/response surface methodology/antioxidant activity分类
农业科技引用本文复制引用
梁志远,甘秀海,杨小生,吴英,黄玉..响应面法优化干花豆总黄酮提取工艺研究[J].广西植物,2016,36(9):1119-1125,7.基金项目
贵州省应用化学特色重点学科(黔教科研发[2012]442号)；贵州省教育厅特色重点实验室项目(黔科合KY[2012]005号)[Suppor-ted by the characteristic key discipline of applied chemistry in Guizhou Province (2012-442)；the Characteristic Key Laboratory Project of Education De-partment in Guizhou Province (2012-005)]。 （黔教科研发[2012]442号）
