化工进展2016，Vol.35Issue(9)：2766-2773,2774,9.DOI:10.16085/j.issn.1000-6613.2016.09.019
粗纤维素酶液对微晶纤维素酶解的影响因素
Influence factors of microcrystalline cellulose hydrolysis by crude cellulase solution
摘要
Abstract
Melanoporia castaneawas often used as laccase-producing strain,but little study was reported about the production of cellulase by this strain. In order to reduce the cost of cellulase in the hydrolysis of cellulose,the crude cellulase solution prepared by the fermentation of Melanoporia castanea. Then microcrystalline cellulose(MCC)was used as a model substrate,and the conditions of microcrystalline cellulose hydrolysis by crude cellulase solution was optimized,including pH,temperature,and type and concentration of surface active agents. Then crude cellulase solution hydrolysis kinetics,and ultraviolet and fluorescence spectrum were studied with the surfactant agents. The results showed that the optimum hydrolysis conditions of microcrystalline cellulose with crude cellulase solution were pH 4.8,temperature 50℃,and surfactant Tween-80 at dosage of 1.12mg/g substrate. It demonstrated that Tween-80 addition could improve the maximum reaction velocity constantsVmax of crude cellulase solution and reduce Michaelis constant(Km). Both maximum absorbance peaks of ultraviolet and fluorescence were changed after addition of surfactants,with amideⅠandⅢ bands infrared spectrum changed,showing that the aromatic amino acid residues in cellulose were reacted with surfactants and then influenced the hydrolysis of microcrystalline cellulose. The study provides a theoretical guide for further reducing the cost of cellulose hydrolysis.关键词
纤维素酶/微晶纤维素/表面活性剂/生物催化/生物质Key words
cellulase/microcrystalline cellulose/surfactants/biocatalysis/biomass分类
轻工纺织引用本文复制引用
沙如意,Edwin Menledy Gbor JR,楼坚,蔡成岗,毛建卫,刘士旺..粗纤维素酶液对微晶纤维素酶解的影响因素[J].化工进展,2016,35(9):2766-2773,2774,9.基金项目
国家级星火计划（2014GA690036、2015GA690057、2015GA700079）、浙江省重点研发计划（2015C02031）、浙江省教育厅科研项目（Y201327551、Y201224264）及浙江科技学院科研启动基金（F501103C01）项目。 （）
