化工进展2016，Vol.35Issue(9)：2987-2996,10.DOI:10.16085/j.issn.1000-6613.2016.09.048
活性黑5的生物脱色、复色现象及机理
Decolorization and repigmentation of reactive black 5 biodegradation and their mechanisms
摘要
Abstract
Wastewater from printing and dyeing industrials is still a severe problem,and biological treatment is the most widely used method currently,for which screening high efficient dye biodegradation microorganisms is the key. In this paper,by using the concentration gradient pressure screening method,we selected a mixed bacterial flora DDMY1,which has good decolorization performance to reactive black 5(RB5),from the well-running hydrolytic acidification reactor for subsequent printing and dyeing wastewater treatment. The bacterial flora was used to study the RB5 decolorization under the facultative-aerobic condition. Firstly,photos were taken in the process of decolorization and repigmentation to record the repeated results visually. Secondly,ultraviolet-visible spectrophotometry was used to scan the decoloring liquid at different times and different states. At last, GC-MS(Gas Chromatography-Mass Spectrometer)and FTIR(Fourier Transform Infrared Spectroscopy)were employed to analyze the degradation products. Results indicated that,the mixed flora DDMY1 performed satisfatorily in decoloring of RB5,and with 400mg/L RB5 for 24h cultivation,the decolorization rate could reach 97.4%. At the same time,the study found that the biological oxygen decolorization reaction could effectively repeat the decolorization-repigmentation for 17 times. According to the analysis results of the tests,it could be speculated that RB5 degradation products such as aniline,naphthoquinones might be responsible.关键词
混合菌群/染料脱色/活性黑5/氧化还原反应Key words
bacterial consortium/biological decoloring/reactive black 5/redox分类
资源环境引用本文复制引用
俞承志,谢学辉,郑秀林,徐乐铱,李然,柳建设..活性黑5的生物脱色、复色现象及机理[J].化工进展,2016,35(9):2987-2996,10.基金项目
国家自然科学基金（21377023，51508083）、中央高校基本科研业务费专项资金（2232015D3-22）及上海市重点学科建设项目（B604）。 （）
