化工学报2016，Vol.67Issue(9)：3523-3535,13.DOI:10.11949/j.issn.0438-1157.20160203
亲和仿生层析及在抗体纯化中的应用
Affinity biomimetic chromatography and its applications for antibody purification
摘要
Abstract
Affinity biomimetic chromatography is a novel bioseparation technology for the biological active substance, particularly showing good performance for antibody purification with the advantages of low cost, stable ligand structure, high specificity and so on. The critical point of affinity biomimetic chromatography is the specially-designed biomimetic ligand. The biomimetic ligands used currently include chemical synthetic ligand and short peptide ligand, which are normally obtained by rational design and screening. For a particular target protein, instead of selecting the ligand randomly, it is necessary to build a reasonable ligand library by rational designing method and screen the suitable mimetic ligand by high-throughput screening technology. According to the recent advances in affinity biomimetic chromatography, the designing and screening of ligands used for affinity mimetic chromatography and the applications in antibody purification are reviewed in the present article.关键词
亲和仿生层析/化学合成配基/短肽配基/抗体/纯化/分子模拟Key words
affinity biomimetic chromatography/chemical synthetic ligand/short peptide ligand/antibody/purification/molecular simulation分类
化学化工引用本文复制引用
卢慧丽,林东强,姚善泾..亲和仿生层析及在抗体纯化中的应用[J].化工学报,2016,67(9):3523-3535,13.基金项目
国家自然科学基金项目（21576233，21276228）。@@@@ supported by the National Natural Science Foundation of China(21576233,21276228) （21576233,21276228）
