黑龙江农业科学Issue(8)：19-22,4.DOI:10.11942/j.issn1002-2767.2016.08.0019
纳米氧化锌消毒小蓟外植体应用研究
Application of Nano-ZnO in Explant Sterilization of Cirsium setosum
摘要
Abstract
In order to achieve the best disinfection effect of nanometer zinc oxide (Nano‐ZnO) in tissue culture , the explants of thistle leaves as materials ,the disinfecting system of Nano‐ZnO was established .Thistle ex‐plants disinfection effect and browning rate gradually rised ,under 1~7 g·L‐1 Nano‐ZnO .The best disinfection concentration of nano‐ZnO was 5 g·L‐1 and the best infection time was 20 min .Under 2 g·L‐1 Nano‐ZnO+0 .1% mercuric chloride condition ,thistle explants had about 20% pollution and callus grew well .3 g·L‐1 of nano zinc oxide + 0 .1% mercuric chloride condition could achieve the best disinfection ,but the callus induc‐tion was inhibited and grow slowly .关键词
纳米氧化锌/小蓟/组织培养/杀菌Key words
nanometer zinc oxide/Cirsium setosum/tissue culture/sterilization分类
农业科技引用本文复制引用
邓凯元,朱雨荷,陈婷,潘玉欣..纳米氧化锌消毒小蓟外植体应用研究[J].黑龙江农业科学,2016,(8):19-22,4.基金项目
华北理工大学在校大学生创新创业训练计划资助项目 （）
