黑龙江农业科学Issue(8)：51-53,54,4.DOI:10.11942/j.issn1002-2767.2016.08.0051
芒果炭疽菌致病力室内快速测定方法研究
A Rapid Laboratory Determining Methods of the Pathogenicity of Mango Anthracnose Pathogen
摘要
Abstract
In order to set up a fast and stable laboratory determining methods for the virulence of the pathogens of mango anthracnose pathogen in China .As with mango anthracnose pathogen strains tested ,the mango an‐thracnose pathogen strains was respectively inoculated on excised twigs ,leaves and fruits ,which were collected from Guifei mango and wounded by different methods .The inoculated materials were cultured at 25 ℃ and the pathogenic severity of all treatments was observed .The results showed that in inoculated twigs ,the lesion lengths of the twigs wounded by scald holing ,holing treatments were significantly bigger than the other inocu‐lated twigs ,and there were significant differences among them .In inoculated leaves ,the lesion lengths of the leaves upper and under side wounded by scalding and pricking ten times were significantly bigger than three times ,six times ,and ahearing .In inoculated fruits ,the lesion lengths of the fruits wounded by scald holing , scalding ,holing and ten times pricking treatments were significantly bigger than three times ,six times pricking , and around ahearing .And there were significant differences among them .Summing up all the factors of the difficulty level of the material choice ,the size of the lesion ,the degree of difficulty ,the test operations and the other stability analysis .The lesion lengths of 5 mm diameter bacteria piece inoculated in ten times pricking in‐juries bronze opposite leaves ,a relative humidity of 100% lesion diameter after 5 d ,at 25 ℃ ,could be used as a basis for evaluation of pathogenic pathogens .关键词
芒果炭疽菌/致病力/室内测定Key words
mango anthracnose pathogen/pathogenicity/laboratory determination分类
农业科技引用本文复制引用
张蕊,杨石有,刘晓妹,蒲金基,张贺..芒果炭疽菌致病力室内快速测定方法研究[J].黑龙江农业科学,2016,(8):51-53,54,4.基金项目
海南省自然科学基金资助项目（20163063）；公益性行业（农业）科研专项资助项目（201203092-2）；海南大学应用科技学院基金资助项目 （）
