黑龙江农业科学Issue(9)：1-5,6,6.DOI:10.11942/j.issn1002-2767.2016.09.0001
垂枝樱花组培技术研究
Study on Tissue Culture of Cerasus subhirtella Miq .var .pendula Tanaka
摘要
Abstract
In order to establish more efficient tissue culture system ,using the stems of Cerasus subhirtella Miq . var .pendula Tanaka as the explants ,the effects of different sterilize time ,starting culture ,proliferation culture and rooting culture were studied .The results showed that the procedures of surface sterilization was 75% et‐hand soak for 30 s ,0 .1% HgCl2 immerse for 10 min ,the sterile water rinse 5 times ,the survival rate of 66 .7% .The suitable inducing medium was :1/2 MS+6‐BA 0 .5 mg·L‐1 + NAA 0 .1 mg·L‐1 ,the differentia‐tionrate reached 68 .3% ,the proliferation medium was MS + 6‐BA 0 .3 mg·L‐1 + NAA 0 .05 mg·L‐1 + GA3 10 mg·L‐1 ,and the proliferation coefficient was 7 .0 ,the average height was 2 .9 cm ,the root inducing medium was 1/2 MS+NAA 1 .0 mg·L‐1 +IBA 0 .5 mg·L‐1 +20 g·L‐1 sucrose and the rooting rate could reach 93% .关键词
垂枝樱花/离体培养/表面灭菌/增殖/生根Key words
Cerasus subhirtella Miq .var .pendula Tanaka/culture medium/surface sterilization/proliferation/rootage分类
农业科技引用本文复制引用
王红梅,李园莉,李洪光,董晓辉,孙艳艳..垂枝樱花组培技术研究[J].黑龙江农业科学,2016,(9):1-5,6,6.基金项目
2015江苏省大学生实践创新训练计划资助项目（201513103020X ）；2012江苏农林职业技术学院科技资助项目 （）
