黑龙江农业科学Issue(9)：11-14,4.DOI:10.11942/j.issn1002-2767.2016.09.0011
野生湖北百合不定芽诱导及再生植株的建立
Study on Adventitious Buds Induction and Plant Regeneration of Wild Liliumhenry i
摘要
Abstract
In order to strengthen the preservation and utilization of wild lilyresources in Hubei province ,using bulb and stem of wild Liliumhenryias explant ,the effect of different hormone combination on adventitious buds induction ,bud proliferation and rooting were studied .The results showed that the optimal medium for adventi‐tious buds induction was MS+1 .0 mg·L‐1 6‐BA+0 .5 mg·L‐1 NAA .The induction rate of stem was higher than bulbs .The rate of induction was 66 .67% and 59 .63% .The bud from single explant was 2 and 1 ,the rate of contamination from stem was lower than bulb .The best proliferation medium was MS + 0 .1 mg·L‐1 6‐BA+1 .0 mg·L‐1 NAA .The rate of proliferation was 83 .33% ,the average of coefficient of proliferation was 2 .11 and the growth was the better .The medium for rooting was 1/2MS+0 .3 mg·L‐1 NAA .The rate of roo‐ting was as high as 93 .33% ,the average number of rooting was 3 .18 .关键词
湖北百合/鳞片/茎段/组织培养Key words
Liliumhenryi/bulb/stem/tissue culture分类
农业科技引用本文复制引用
蒋瑶,陈文波,周朝伟,陈其兵..野生湖北百合不定芽诱导及再生植株的建立[J].黑龙江农业科学,2016,(9):11-14,4.基金项目
贵州省科技厅资助项目（黔科合 J 字［2014］2153号）；凯里学院博士启动基金资助项目 （）
