黑龙江农业科学Issue(9)：15-18,4.DOI:10.11942/j.issn1002-2767.2016.09.0015
大穗结缕草愈伤组织诱导及植株再生研究
Study on Callus Induction and Plant Regeneration of Zoysia macrostachy a Franchet .Sav
摘要
Abstract
In order to identify the best conditions on rapid propagation of Zoysia macrostachya ,together with MS as basic medium ,different methods of disinfection of explants were studied ,the effect of the different types explants (seeds ,leaves of Z .macrostachya as explants) and different hormone combinations on induction and differentiation of callus was researched .The results showed that in the condition of light intensity was 1 500~2 000 lx for 16 h light /8 h dark ,(23 ± 2)℃ ,disinfected with being immersed in 70% alcohol for 45 s ,being washed with aceptic water 3 times ,being immersed in 0 .1% HgCl2 for 8 min ,being washed with aceptic water 3 times ,seed embryo are easy to callus growth with low contamination rate .Using seed embryo as explant ,the best induction medium were MS+2 ,4‐D(2 .0 mg·L‐1 )+6‐BA(0 .2 mg·L‐1 ) + TDZ(1 .0 mg·L‐1 ) .关键词
大穗结缕草/愈伤组织/植株再生Key words
Zoysia macrostachya/callus/plant regeneration分类
农业科技引用本文复制引用
马龙雪,赵丽萍..大穗结缕草愈伤组织诱导及植株再生研究[J].黑龙江农业科学,2016,(9):15-18,4.基金项目
国家大学生创新训练资助项目（201410449004）；山东省自然科学基金资助项目（2010ZRA16022）；滨州学院科研基金资助项目 （）
