湖泊科学2016，Vol.28Issue(5)：1148-1152,5.DOI:10.18307/2016.0526
浮游植物叶绿素a的微波法研究及其与反复冻融法的比较
Microwave-assisted method for extracting chlorophyll-a in phytoplankton and its compari-son with freezing-thawing extraction method
摘要
Abstract
In order to save time of sample processing and to improve the determining accuracy, a microwave-assisted extraction method was designed and optimized to extract chlorophyll-a in phytoplankton, and was compared to the freezing-thawing method for extraction efficiency of chlorophyll-a. The results showed that microwave-assisted extraction method was optimal in 60 s treatment with output power of 800 W, and the appropriate water volume for filtration was about 100-500 ml for samples collected in meso-eutrophic waterbodies and 1000 ml for those from oligotrophic waterbodies. The chlorophyll extraction rate of microwave-assisted ex-traction method was significantly higher than that of freezing-thawing method in meso-eutrophic waterbodies, which can increased by 7%-12%, while freezing-thawing method was more stable in measuring chlorophyll-a of oligotrophic waterbodies. For those al-gae with a glue shell or siliceous shell, microwave-assisted extraction method had a higher efficiency for chlorophyll-a extraction and a lower relative standard deviation than the freeze-thaw method. The microwave-assisted extraction method needs only one half oper-ation time of the freezing-thawing extraction method, so that it is more suitable for emergency monitoring of eutrophic waterbodies, while the freezing-thawing extraction method is more suitable for monitoring oligotrophic waterbodies.关键词
微波法/反复冻融法/提取/浮游植物/叶绿素aKey words
Microwave-assisted extraction method/freezing-thawing method/extraction/phytoplankton/chlorophyll-a引用本文复制引用
林罗敏,唐鹊辉,彭亮,韦桂峰,林叔忠,李湘姣,杨浩文..浮游植物叶绿素a的微波法研究及其与反复冻融法的比较[J].湖泊科学,2016,28(5):1148-1152,5.基金项目
广东省水利科技创新项目“广东省河流水生态健康评价指标体系及评价方法研究”（2014-01）资助 （）
