江西农业学报2016，Vol.28Issue(9)：5-8,13,5.DOI:10.19386/j.cnki.jxnyxb.2016.09.02
绵羊卵泡液高丰度白蛋白去除方法的比较
Comparative Study on Effects of Removing High-abundance Albumi n from Sheep Follicular Fluid by Two Different Kits
摘要
Abstract
We compared the effects of removing high-abundance albumin from sheep follicular fluid by two different kits .The results showed that PierceTM Albumin Depletion Kit had a high efficiency of removing high-abundance albumin, but its specificity was poor.ProteoExtract ® Albumin/IgG Removal Kit had a lower efficiency of removing high-abundance albumin than the for-mer, and it could not effectively remove the IgG from sheep follicular fluid , but it had a better specificity in removing from albu-min than the former.The 2-DE electrophoresis patterns showed that the coloration degree and area of albumin district of sheep follicular fluid samples treated with two kits were smaller than those of untreated samples .PierceTM kit had a low recovery efficien-cy to macromolecular and low-abundance proteins, while ProteoExtract ® kit had a low recovery efficiency to small-molecular and low-abundance proteins.In the protein diagram of the samples treated with Pierce TM kit, there were more detected points.关键词
绵羊/卵泡液/白蛋白/去除方法Key words
Sheep/Follicular fluid/Albumin/Removing method分类
农业科技引用本文复制引用
陈莹,李晓林,林嘉鹏,吴阳升,黄俊成..绵羊卵泡液高丰度白蛋白去除方法的比较[J].江西农业学报,2016,28(9):5-8,13,5.基金项目
国家自然科学基金项目（U1203381）；新疆维吾尔自治区重点实验室开放课题（2015KL007）。 （）
