检验医学与临床2016，Vol.13Issue(18)：2582-2584,3.DOI:10.3969/j.issn.1672-9455.2016.18.011
某地区乙型肝炎病毒基因型分布和P区耐药突变模式分析
Distribution of hepatitis B virus genotypes and drug resistance mutations in P region in a certain area
左万超 1刘小亮 2李莲 1付红霞3
作者信息
- 1. 湖北医药学院附属人民医院检验部，湖北十堰 442000
- 2. 湖北省丹江口市汉江医院药剂科 442700
- 3. 湖北省十堰市太和医院检验科 442000
- 折叠
摘要
Abstract
Objective To study the distribution of hepatitis B virus genotypes in Shiyan City and analyse the correlation between it and the pattern of drug resistance mutation in P district .Methods Totally 224 patients with chronic hepatitis B were enrolled in the study ,after the HBV DNA samples were extracted ,nucleotide sequencing was carried out by using ABI 3730 genetic analyzer , the sequence alignment was done by using SeqMan software .The HBV genotype and drug resistance mutation at P region of HBV DNA were analyzed .Results Genotype B was detected in 136 cases accounting for 60 .71% ;genotype C was detected in 62 cases accounting for 27 .68% .The proportion of the two were higher than those of D type ,B + C type and non‐classification cases ,the difference was statistically significant(P< 0 .05) .Totally 78 cases were confirmed to have drug resistance mutation .The drug re‐sistance rate of genotype B to lamivudine and adefovir were 18 .38% and 9 .56% respectively ,while those of genotype C were 16 .13% and 9 .68% respectively .The difference between the two genotypes was not statistically significant(P> 0 .05) .There was no significant difference among the proportion of B mutations(M204V ,M204I) and non YMDD mutation in patients(P> 0 .05) .B and C genotypes were positively correlated with M204V and M204I mutations .Conclusion The genotypes of HBV in Shiyan city were mainly B and C and the types of drug resistance mutations were mainly M204V and M204I .The therapies should be chosen ac‐cording to the genotype and drug resistance mutation .关键词
乙型肝炎病毒/基因型/耐药突变/相关性Key words
hepatitis B virus/genotype/drug resistance mutations/correlation引用本文复制引用
左万超,刘小亮,李莲,付红霞..某地区乙型肝炎病毒基因型分布和P区耐药突变模式分析[J].检验医学与临床,2016,13(18):2582-2584,3.
