检验医学与临床2016，Vol.13Issue(18)：2588-2589,2592,3.DOI:10.3969/j.issn.1672-9455.2016.18.013
糖尿病肾病几种试验联合检测用于诊断的探讨
Analysis on several kinds of combined detection and diagnosis in diabetic nephropathy
杨辛 1吴颖稚 1钱重玲 1范君 1林洁 1李菁 1张庆五1
作者信息
- 1. 上海市杨浦区控江医院检验科 200093
- 折叠
摘要
Abstract
Objective To investigate the clinical values of urinary microalbumin(mAlb) ,serum cystatin C (Cys‐C) ,retinol bind‐ing protein (RBP) and dodecyl sulfosalicylic acid sodium‐agarose gel (SDS‐AGE) electrophoresis in diagnosis of diabetic nephropa‐thy(DN) .Methods Urinary mAlb and serum RBP levels were detected in 148 cases of clinically diagnosed DN by using the im‐mune turbidity method and serum Cys‐C level was simultaneously detected by using latex enhanced immunoassay and SDS‐AGE e‐lectrophoresis .Results Urine mAlb ,serum Cys‐C and RBP levels had statistical differences between the DN group and the control group(P< 0 .05) .In the evaluation of various urinary protein groups according to the urinary protein compositions and molecular weight of proteins after SDS‐AGE electrophoresis ,98 cases (66 .2% ) were glomerular proteinuria ,6 cases (4 .1% ) were renal tubu‐lar proteinuria ,29 cases (19 .6% ) were mixed type proteinuria and 15 cases (10 .1% ) were false positive proteinuria .In the compar‐ison of the positive results of urinary mAlb (82 .7 ± 12 .5)mg/L ,serum Cys‐C (1 .85 ± 0 .58)mg/L and serum RBP (108 .00 ± 11 .85) mg/L with the positive rate of urine protein electrophoresis ,among 148 cases of DN ,urinary mAlb was verified as glomeru‐lar proteinuria in 98 cases (66 .2% ) ,renal tubular proteinuria in 4 cases (4 .1% ) ,mixed proteinuria in 29 cases (19 .6% ) and false positive proteinuria in 14 cases (9 .5% ) ,but serum CyS C electrophoresis confirmed glomerular proteinuria in 98 cases (66 .2% ) , renal tubular proteinuria in 4 cases (4 .1% ) and mixed proteinuria accounted in 29 cases (19 .6% ) ;serum RBP electrophoresis con‐firmed mixed proteinuria in 29 cases (19 .6% ) ,renal tubular proteinuria in 4 cases (4 .1% ) .It was found from the contrast of above four kinds of test results that the serum CyS C and RBP had the results same to the SDS‐AGE electrophoresis .Conclusion The SDS‐AGE electrophoresis has higher specificity and detection rate of pathological type of proteinuria ,kidney damage and location judgment and should serve as a diagnostic criterion index of DN .The combined detection of urine mAlb ,serum CyS C and RBP can improve the sensitivity of the early diagnosis of DN and has an important clinical value .关键词
糖尿病肾病/尿微量清蛋白/血清胱抑素C/视黄醇结合蛋白/病理性蛋白尿Key words
diabetes nephropathy/urine albumin/serum cystatin C/retinol binding protein/electrophoresis/patho-logical protein urine引用本文复制引用
杨辛,吴颖稚,钱重玲,范君,林洁,李菁,张庆五..糖尿病肾病几种试验联合检测用于诊断的探讨[J].检验医学与临床,2016,13(18):2588-2589,2592,3.
