内蒙古民族大学学报（自然科学版）2016，Vol.31Issue(4)：321-325,5.DOI:10.14045/j.cnki.15-1220.2016.04.011
人重组5-脂氧合酶基因真核表达载体的构建和鉴定
Reconstructing and Identifying Eukaryotic Expression Vectors of Gene 5-Lipoxygenase
摘要
Abstract
Ideas and constructing methods of human 5-Lipoxygenase eukaryotic expression vectors sre presented and the expression of 5-Lipoxygenase in HEK293T cells by transfection and western blotting analysis is identified.关键词
5-脂氧合酶基因/pcDNA3.1(+)/载体/RT-PCR/Western blotting/蛋白表达Key words
Gene 5-lipoxygenase/PcDNA3.1(+)/Vector/RT-PCR/Western blotting/Protein expression分类
医药卫生引用本文复制引用
白春英,史铁伟,瑞云,李天柱..人重组5-脂氧合酶基因真核表达载体的构建和鉴定[J].内蒙古民族大学学报（自然科学版）,2016,31(4):321-325,5.基金项目
国家自然科学基金项目资助 （）
