日用化学工业2016，Vol.46Issue(8)：427-432,6.DOI:10.13218/j.cnki.csdc.2016.08.001
多季铵盐与鲑鱼精 DNA 的相互作用研究
Study on the interaction of multi - quaternary ammonium salt with salmon sperm DNA
摘要
Abstract
The interaction between the multi - quaternary ammonium salt (MQAS)and salmon sperm DNA was investigated using UV - vis absorption spectroscopy,fluorescence spectroscopy,circular dichroism spectroscopy and molecular simulation. The results showed that MQAS binds to salmon sperm DNA by static electricity,and the binding ratio is 5. 5∶ 1. The presence of salmon sperm DNA promotes the formation of MQAS aggregates. With decreasing chain length,the interaction between MQAS and salmon sperm DNA is stronger. In addition,MQAS causes the conformational change of salmon sperm DNA. The results of molecular simulation suggested that MQAS inserts into the minor groove of salmon sperm DNA,and forms stable compound with DNA.关键词
多季铵盐/鲑鱼精DNA/相互作用/非病毒载体Key words
multi - quaternary ammonium salt/salmon sperm DNA/interaction/non - viral vector分类
化学化工引用本文复制引用
李亚萍,赖璐,钟洲,梅平,陈武,刘义..多季铵盐与鲑鱼精 DNA 的相互作用研究[J].日用化学工业,2016,46(8):427-432,6.基金项目
国家自然科学基金青年基金资助项目（21403017）；中国石油科技创新基金资助项目 （）
