日用化学工业2016，Vol.46Issue(8)：479-484,6.DOI:10.13218/j.cnki.csdc.2016.08.011
化妆品中动物源性成分多重实时荧光 PCR检测方法的研究
Study of multiplex real - time fluorescent PCR detection method for animal originated components in cosmetics
摘要
Abstract
DNA of animal originated components in paste,liquid and solid form cosmetic products were sampled using improved Chelex - 100 combined with Glass Milk method. According to the donkey,horse and cattle mitochondrial 16S rRNA gene sequences,the universal primers and specific probes were designed. Then a multiplex real - time fluorescent PCR assay system to detect the species of animal originated (donkey,horse and cattle)components in cosmetic products was established. All the detection limit of all cosmetics achieves 0. 001 ng. Samples from marketed cosmetics such as creams,lotions and facial masks were detected by this way. The reliability and accuracy of this multiplex real - time fluorescent PCR assay were confirmed.关键词
化妆品/多重实时荧光PCR/动物源性成分Key words
cosmetics/multiplex real - time fluorescent PCR/animal originated components分类
化学工程引用本文复制引用
刘艳艳,霍胜楠,梁水美,卞如如,张卉,步迅..化妆品中动物源性成分多重实时荧光 PCR检测方法的研究[J].日用化学工业,2016,46(8):479-484,6.基金项目
济南市高校院所自主创新项目（201401264）；山东省博士基金资助项目 （）
