山东中医杂志2016，Vol.35Issue(10)：909-911,923,4.
白僵蚕体外抗病毒有效部位的筛选
To Determine the Effective in Vitro Antiviral Part of Bombyx Batryticatu
王清 1牛文斐 1张加泽 1侯宝山 1刘玉堂 2周长征3
作者信息
- 1. 山东中医药大学，山东 济南 250355
- 2. 青岛市黄岛区第二中医医院，山东 青岛 266510
- 3. 山东中医药大学药学院，山东 济南 250355
- 折叠
摘要
Abstract
Objective:To explore the in vitro antiviral part of Bombyx batryticatu in macroporous adsorption resin eluent,and after further purification to isolate the antiviral monomer. Methods:After extracted with sol-vent of different polarity,the antiviral effect was observed. Using macroporous adsorption resin method,the supernate of alcohol extraction-water precipitation of Bombyx batryticatu was eluted with distilled water ,25%ethanol,50% ethanol and 75% ethanol,and the eluent was collected. The EV-71-RD cells,RSV,HSV-1-Vero cell transfected with virus to evaluate antiviral effect,and cytopathic effect(CPE) was observed under micro-scope. The neutral red staining A540 value was detected with microplate reader to calculate the therapeutic index(TI). Results:The supernate of the product of alcohol extraction-water precipitation of Bombyx batryt-icatu could suppress respiratory syncytial virus(RSV). Conclusion:The effect part of Bombyx batryticatu in hibiting RSV was in the supernate of the alcohol extraction-water precipitation.关键词
白僵蚕/提取物/醇提水沉法/抗病毒/大孔吸附树脂Key words
Bombyx batryticatu/extract/alcohol extraction-water percipitation/antiviral/macroporous adsorption resin分类
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王清,牛文斐,张加泽,侯宝山,刘玉堂,周长征..白僵蚕体外抗病毒有效部位的筛选[J].山东中医杂志,2016,35(10):909-911,923,4.
