山东农业科学2016，Vol.48Issue(9)：1-5,5.DOI:10.14083/j.issn.1001-4942.2016.09.001
谷子类受体蛋白激酶基因 SiRLK35的克隆及原核表达
Cloning of Receptor -Like Protein Kinase Gene SiRLK35 from Foxtail Millet and Its Prokaryotic Expression
摘要
Abstract
In this research,the full -length sequence of receptor -like protein kinase gene SiRLK35 (NCBI accession number:XM_004956247.2)was amplified by PCR technique with the cDNA of Yugu 1 as template.Bioinformatics analysis showed that the protein product of SiRLK35 was composed of 392 amino acids.It contained one S_TKc conserved domain,one TFS2N domain,three transmembrane domains and a N terminal signal peptide.The sequence of SiRLK35 was digested with BamHⅠ and SalⅠ,and then the recom-bined prokaryotic expression vector pET -28a -SiRLK35 was constructed successfully through connecting the fragment with pET -28a.The recombinant was further transformed into E.coli strain BL21 (DE3).Induced with 0.5 mmol /L IPTG,a 44.6 kD fusion protein was obtained,which proved that the SiRLK35 could be in-duced to express in E.coli.This research layed a good foundation for further study of the function of SiRLK35 gene.关键词
谷子/类受体蛋白激酶/SiRLK35/原核表达Key words
Foxtail millet/Receptor -like protein kinase/SiRLK35/Prokaryotic expression分类
农业科技引用本文复制引用
王一帆,李臻,潘教文,王庆国,刘炜..谷子类受体蛋白激酶基因 SiRLK35的克隆及原核表达[J].山东农业科学,2016,48(9):1-5,5.基金项目
山东省重大科技专项“耐盐碱作物新品种培育改良关键技术研究”（2015ZDGS03001－2）；山东农业大学作物生物学国家重点实验室开放课题“利用 iTRAQ 技术筛选并研究谷子抗旱相关基因的功能” （）
