山东农业科学2016，Vol.48Issue(9)：6-9,16,5.DOI:10.14083/j.issn.1001-4942.2016.09.002
葱属主要蔬菜作物高质量 RNA 提取方法研究
High Quality RNA Extraction Method from Main Allium Vegetables
摘要
Abstract
Allium vegetables (including Allium cepa,Allium fistulosum L.var.giganteum Makion,Alli-um sativum and Allium tuberosum Rottl.ex Spreng)are rich in polysaccharides and other secondary metabo-lites,which seriously affect the quality of extracted RNA.In this study,we compared and analyzed a variety of RNA extraction methods using onion leaves as samples,and obtained a set of method for RNA extraction from Allium vegetables.This method was comprised of CTAB -GT,Proteinase K instead of DEPC and ice bath gel electrophoresis.The yield of RNA samples was about 152.35 μg/gFW,the RNA integrity (RIN val-ue)was about 7.5,the bands of gel electrophoresis were clear without contaminations of polysaccharides,pro-tein and DNA residues,the ratio of 28S /18S was about 2 and the extraction process was safe without strong carcinogen of DEPC.The RNA quality was suitable for downstream molecular experiments.Besides,this method could be applied to the extractions of different tissues of welsh onion,onion,garlic and Chinese chives.关键词
葱属蔬菜/RNA 提取/多糖/CTAB -GTKey words
Allium vegetables/RNA extraction/Polysaccharides/CTAB -GT分类
农业科技引用本文复制引用
孙亚玲,霍雨猛,陈立,刘冰江,缪军,孔素萍,高莉敏,曹辰兴,吴雄,杨妍妍..葱属主要蔬菜作物高质量 RNA 提取方法研究[J].山东农业科学,2016,48(9):6-9,16,5.基金项目
国家自然科学基金项目（31201635、31301786）；山东省农业科学院青年英才重点扶持项目 （）
