山东农业科学2016，Vol.48Issue(9)：127-130,146,5.DOI:10.14083/j.issn.1001-4942.2016.09.029
杂交鹅掌楸总黄酮检测及 DPPH 自由基清除作用
Determination of Total Flavonoids and Their Scavenging Activity on DPPH Free Radicals in Liriodendron Hybrid Leaves
摘要
Abstract
The total flavonoids in Liriodendron hybrid leaves were determined by spectrophotometry using AlCl3 -NaAc chromogenic system.Their contents and DPPH radical scavenging ability were compared with those in other parts.The results showed that the optimum wavelength was 272 nm for determination of total fla-vonoids in Liriodendron hybrid leaves.The optimal determination time was in 30 ~80 minutes after color de-velopment.The relative standard deviation (RSD)of precision test was 0.56% (n =5),and the average re-covery rate was 103.64% (RSD =2.05%),so this method was accurity and reliable.Using this method,the contents of total flavonoids in Liriodendron hybrid leaves was 4.67% (RSD =2.01%,n =5),which was ob-viously higher than those of any other parts.The ability of scavenging DPPH radical of total flavonoids from different parts was in the order of root >leaf >bark >flower >petiole >branches with the IC50 as 6.65,9.88, 10.25,15.55,19.71 and 24.15 μg/mL respectively.In conclusion,the content of total flavonoids in Lirio-dendron hybrid leaves was significantly higher with stronger antioxidant activity,so it had potential value of de-velopment and utilization.关键词
杂交鹅掌楸/总黄酮/含量检测/DPPH 自由基清除能力Key words
Liriodendron hybrid/Total flavonoids/Content determination/DPPH radical scavenging ability分类
农业科技引用本文复制引用
姚文红,李飞阳,孙立梅,张林超..杂交鹅掌楸总黄酮检测及 DPPH 自由基清除作用[J].山东农业科学,2016,48(9):127-130,146,5.基金项目
青岛农业大学大学生科技创新基金项目 （）
