生物技术通报2016,Vol.32Issue(9):100-106,7.DOI:10.13560/j.cnki.biotech.bull.1985.2016.09.014
ABA对低温胁迫下小桐子幼苗耐冷性及甜菜碱积累的影响
Effect of Exogenous Abscisic Acid on Chilling Tolerance and Glycine Betaine Accumulation in Jatropha curcas Seedlings Under Chilling Stress
摘要
Abstract
The objective is to investigate the effect of exogenous abscisic acid(ABA)on chilling tolerance and glycine betaine accumulation of Jatropha curcas seedlings under chilling stress. Seedlings of J. curcas under chilling stress were treated with 150 μmol/L ABA, and the survival rate,root vitality,electrolyte leakage,the content of malondialdehyde(MDA),the content of glycine betaine,and the activity of the key enzyme betaine aldehyde dehydrogenase(BADH)of glycinebataine biosynthesis as well as the expression of JcBADH were measured. The results showed that treatment with 150 μmol/L ABA significantly increased survival rate and root vitality,decreased MDA content and electrolyte leakage in J. curcas seedlings under chilling stress. ABA also enhanced the accumulation of glycine betaine,increased the activity of BADH,and improved the expression level of the JcBADH. The results suggested that exogenous ABA treatment may enhance chilling tolerance in J. curcas seedlings,and glycine betaine might play a key role in the increasing of chilling tolerance.关键词
脱落酸/低温胁迫/甜菜碱/耐冷性/小桐子Key words
abscisic acid/chilling stress/glycine betaine/chilling tolerance/Jatropha curcas引用本文复制引用
杨双龙,邓凤飞,杜朝昆,成蕴秀,龚明..ABA对低温胁迫下小桐子幼苗耐冷性及甜菜碱积累的影响[J].生物技术通报,2016,32(9):100-106,7.基金项目
国家自然科学基金项目(31260169,31260064,31460059),云南师范大学研究生科研创新基金项目 ()