生物技术通报2016，Vol.32Issue(9)：107-113,7.DOI:10.13560/j.cnki.biotech.bull.1985.2016.09.015
西瓜枯萎病拮抗链霉菌分离鉴定及盆栽抗病试验
Isolation and Identification of Antagonistic Streptomyces spp. Against Fusarium Wilt of Watermelon and Primarily Potted Inhibition Test
摘要
Abstract
The objective is to select a natural antagonistic bacterium efficiently controlling Fusarium oxysporum f. sp. niveum that is the pathogenic bacterium of the fusarium wilt of watermelon. Using the method of gradient dilution to isolate actinomycetes from rhizosphere soil of continuous cropping pepper,it was purified and the plate confrontation method was applied to select an antagonistic strain. Then the method of basin nursery substrates mixed with bacteria was for inoculation,a potted plant disease-resistant experiment was carried out,and the strains with the optimal antagonistic effects were identified. The results showed that 10 strains of actinomycetes with the antagonistic effects to fusarium wilt of watermelon were isolated from the growing pepper rhizosphere soil,and 3 of them(F22,F23 and F32)presented the significantly antagonistic effects. After 96 hours the diameter of the inhibition zone was 1.70 cm,1.17 cm and 1.47 cm respectively. The result of the potted plant experiments demonstrated that the control effect of the antagonistic strain F32 to the fusarium wilt of watermelon reached maximum with 66.7% at 30 d,moreover,it also had a wide antibacterial spectrum. Based on the phenotypic characteristics,physiological-biochemical characteristics,and the results of 16S rDNA molecular analysis,the F32 was identified as Streptomyces sp.,and F32 presented solid control effects on the fusarium wilt of watermelon,thus it is biocontrol strain with potential application value.关键词
西瓜枯萎病/尖孢镰刀菌/拮抗菌/生物防治Key words
fusarium wilt of watermelon/actinomycetes/antagonistic microorganism/biological control引用本文复制引用
任迁琪,宋星林,吴洪生,李季,陈素云,徐亚,姚东良,赵月,陈健,李美颖..西瓜枯萎病拮抗链霉菌分离鉴定及盆栽抗病试验[J].生物技术通报,2016,32(9):107-113,7.基金项目
江苏省研究生培养创新工程项目（KYLX_0851），江苏省大学生实践创新计划 （）
