生物技术通报2016，Vol.32Issue(9)：156-164,9.DOI:10.13560/j.cnki.biotech.bull.1985.2016.09.021
中华大蟾蜍γ-肌动蛋白基因 Bbgγ-Actin 的克隆与生物信息学分析
Cloning and Bioinformatic Analysis of γ-Actin Gene(Bbgγ-Actin)from Bufo bufo gargarizans
摘要
Abstract
In order to obtain the full-length of actin’s sequence in Bufo bufo gargarizans,the total RNA was extracted from the parotid gland of B. bufo gargarizans,and high-throughput sequencing of transcriptome was used to obtain the full-length cDNA of actin(BbgActin)in B. gargarizansas 2 055 bp. The sequence of ORF(open reading frame)was verified by RT-PCR and sequencing. Sequence analysis showed that the ORF was 1 131 bp encoding for a polypeptide of 376 amino acid residues. Homologous alignment by software BLAST P revealed that it shared over 89% of nucleotide identities and over 90% of amino acid identities with actins from other animals in GenBank. Phylogenetic analysis showed that BbgActin clustered in a class with γ-Actin and highly conserved during evolution. In conclusion,it is the first time to acquire the full-length sequence of γ-Actin cDNA in B. bufo gargarizans,which lays a foundation for further studies on the role of gene Bbgγ-Actin on the life activities of B. bufo gargarizans.关键词
中华大蟾蜍/γ-肌动蛋白/cDNA 全长/系统进化Key words
Bufo bufo gargarizans/γ-Actin/full-length of cDNA/system evolution引用本文复制引用
刘彦芳,王秋爽,凌鸿,刘东春,代英辉,王东..中华大蟾蜍γ-肌动蛋白基因 Bbgγ-Actin 的克隆与生物信息学分析[J].生物技术通报,2016,32(9):156-164,9.基金项目
辽宁省自然科学基金项目（2015020741），沈阳药科大学归国人员科研启动基金项目 （）
