生物技术通报2016，Vol.32Issue(9)：189-196,8.DOI:10.13560/j.cnki.biotech.bull.1985.2016.09.025
绿色木霉耐热β-葡萄糖苷酶分离纯化及酶学性质研究
Separation,Purification,and Enzymatic Properties of Thermo-tolerant β-glucosidase from Tridchoderma viride
摘要
Abstract
This study aims to separate and purify the β-glucosidase of Tridchoderma viride and to investigate its enzyme characterization. T. viride GIM3.139 was fermented in shaking flask,and the enzymatic properties of the purified β-glucosidase by centrifugal ultrafiltration and Sephadex G-200 gel chromatography were studied. Further,the separated and purified constituents were tested by PAGE(polyacrylamide gel electrophoresis),and it reached the electrophoretic-purity. The results showed that the optimal reaction temperature was 80℃ and it was still in a high enzymatic activity for a long time in the range of 75-95℃ ;also the β-glucosidase presented a strong stability under acidic and alkaline conditions and its optimal pH value was 6.5. Some metal ions including Fe3+,Mg2+,and K+ inhibited the activity of β-glucosidase,among which Fe3+ did it the most significantly. However,Fe2+ and Mn2+ activated the enzyme,and Mn2+ did it the most significantly. Organic solvents such as methanol and acetone increased the enzymatic activity,and methanol did it the most significantly. However,the ethyl acetate showed obvious inhibition effect. In conclusion,electrophoretic-purity β-glucosidase was separated and purified from T. viride and the enzymatic properties were characterized as well.关键词
绿色木霉/β-葡萄糖苷酶/分离纯化/酶学性质Key words
Tridchoderma viride/β-glucosidase/separation and purification/enzymatic properties引用本文复制引用
彭利沙,张永祥,闫青,王翔,李军..绿色木霉耐热β-葡萄糖苷酶分离纯化及酶学性质研究[J].生物技术通报,2016,32(9):189-196,8.基金项目
国家自然科学基金项目（31570374），河北省自然科学基金项目 （）
