中国组织工程研究2016，Vol.20Issue(36)：5351-5357,7.DOI:10.3969/j.issn.2095-4344.2016.36.005
AMD3100联合地塞米松对造血干细胞的动员作用
Combination effect of AMD3100 and dexamethasone on the mobilization of hematopoietic stem cells
摘要
Abstract
BACKGROUND:The number of hematopoietic stem cel s in the peripheral blood is very low at normal physiological state. So it is critical to mobilize hematopoietic stem cel s from donor’s bone marrow into the peripheral blood. <br> OBJECTIVE:To study the combination effect of AMD3100 and dexamethasone on the mobilization of hematopoietic stem cel s in mice, thereby laying the foundation for clinical application. <br> METHODS:C57BL/6 mice were injected with AMD3100 and dexamethasone alone or in combination. Then, hematopoietic stem cel s in the peripheral blood and bone marrow were col ected. CD34+cel concentration in the peripheral blood and bone marrow was determined by flow cytometer and the content of leucocytes in the peripheral blood was counted by a normal method. The CFU-Mix colony formation ability of hematopoietic stem cel s was identified by cel colony forming assay. <br> RESULTS AND CONCLUSION:The concentration of CD34+cel s in the peripheral blood and bone marrow, the content of leukocytes in the peripheral blood and the CFU-Mix colony formation ability of hematopoietic stem cel s were al improved by both AMD3100 and dexamethasone and especial y their combined use. This indicates that both AMD3100 and dexamethasone could mobilize hematopoietic stem cel s to migrate from the bone marrow to the peripheral blood, and when used in combination, the mobilization effect is better than that of single drug.关键词
干细胞/造血干细胞/地塞米松/AMD3100/造血干细胞移植/动员分类
医药卫生引用本文复制引用
燕备战,马会敏,孔存权,梁玉,朱伟彦,蒋舒婷..AMD3100联合地塞米松对造血干细胞的动员作用[J].中国组织工程研究,2016,20(36):5351-5357,7.基金项目
河南省科技攻关项目(142102310126)Funding:the Scientific Tackle Key Project of Henan Province, China, No.142102310126 （142102310126）
