中国组织工程研究2016，Vol.20Issue(36)：5371-5377,7.DOI:10.3969/j.issn.2095-4344.2016.36.008
骨髓间充质干细胞移植治疗老年性痴呆模型大鼠的行为学检测
Bone marrow mesenchymal stem cell transplantation improves behavior performance of senile dementia rats
摘要
Abstract
BACKGROUND:More recently, studies have demonstrated that bone marrow mesenchymal stem cel s can be induced in vitro to differentiate into neuron-like cel s that are used for in vivo transplantation to repair nerve damage. <br> OBJECTIVE:To study the effect of bone marrow mesenchymal stem cel transplantation on learning and memory ability of senile dementia rats. <br> METHODS:Thirty male Sprague-Dawley rats were randomly divided into three groups:normal control group, stem cel therapy group and model control group. Rats in the latter two groups were used to establish animal models of senile dementia by intracranial injection ofβ-amyloid 1-40. Three weeks after modeling, rats were given bilateral hippocampal injection of induced bone marrow mesenchymal stem cel suspension in the stem cel therapy group, whereas no treatment was given in the normal control and model control groups. Morris water maze test was used to detect learning and memory ability of rats, and rat’s brain tissues were detected pathological y using hematoxylin-eosin staining. <br> RESULTS AND CONCLUSION:After modeling, the escape latency was higher and the cross-platform frequency was lower in the model control group compared with the normal control group. After cel transplantation, the escape latency and cross-platform frequency were gradual y shortened and increased with time, respectively. Compared with the model control group, the learning and memory abilities of rats were improved in the stem cel therapy group. The brain tissues were relatively intact in structure and exhibited less cel degeneration and necrosis in the stem cel therapy group compared with the model control group. To conclude, bone marrow mesenchymal stem cel transplantation exerts certain therapeutic effects on senile dementia by effectively improving the learning and memory ability.关键词
干细胞/移植/骨髓间充质干细胞/老年性痴呆/学习记忆/大鼠分类
医药卫生引用本文复制引用
张颖,郭建华,闫洪娟,罗秋华,李鑫萍,郭玮,王海霞..骨髓间充质干细胞移植治疗老年性痴呆模型大鼠的行为学检测[J].中国组织工程研究,2016,20(36):5371-5377,7.基金项目
河北省医学科学研究重点课题(20130921)Funding:the Medical Research Project of Hebei Province, No.20130921 （20130921）
