中国组织工程研究2016，Vol.20Issue(37)：5483-5488,6.DOI:10.3969/j.issn.2095-4344.2016.37.002
中药川芎对成骨细胞作用最佳浓度的体外实验
Effects and optimum concentration of Rhizoma Chuanxiong on osteoblasts in vitro
鲁巍 1郭睿 1赵刚1
作者信息
- 1. 佳木斯大学口腔医学院，黑龙江省佳木斯市 154002
- 折叠
摘要
Abstract
BACKGROUND:It has been proved that Rhizoma Chuanxiong can promote osteoblast proliferation and differentiation. <br> OBJECTIVE:To further observe the effects of tetramethylpyrazine (Ligustrazine) extracted from Rhizoma Chuanxiong on osteoblast proliferation and type I col agen expression. <br> METHODS:Mouse osteoblast cel line MC3T3-E1 was cultured in medium containing 1, 5, 10, 15 and 20 mg/L Ligustrazine, respectively. Osteoblast proliferation was detected by cel counting kit-8 assay, and alkaline phosphatase activity and type I col agen level measured using ELISA method. <br> RESULTS AND CONCLUSION:Different concentrations of Ligustrazine al significantly promoted the osteoblast proliferation and type I col agen level compared with the blank control group (P<0.005). The activity of alkaline phosphatase in al Ligustrazine groups except 15 and 20 mg/L groups was significantly higher than that in the blank control group (P<0.005). These results show that Ligustrazine isolated from Rhizoma Chuanxiong can effectively promote the osteoblast proliferation and type I col agen expression, with the optimum concentration of 10 mg/L.关键词
组织构建/骨细胞/川芎嗪/成骨细胞/细胞增殖/碱性磷酸酶/Ⅰ型胶原蛋白分类
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鲁巍,郭睿,赵刚..中药川芎对成骨细胞作用最佳浓度的体外实验[J].中国组织工程研究,2016,20(37):5483-5488,6.
