中国组织工程研究2016，Vol.20Issue(37)：5504-5510,7.DOI:10.3969/j.issn.2095-4344.2016.37.005
体外震波对骨微血管内皮细胞生理功能的影响
Impact of extracorporeal shock waves on physiological function of bone microvascular endothelial cells
左伟 1高福强 1李配瑶 1孙伟 1李子荣 1时令军1
作者信息
摘要
Abstract
BACKGROUND:Extracorporeal shock wave has been shown to influence the physiological function of endothelial cel s via the activation of mechanoreceptors and specific signal transduction system, and gene expression regulation. <br> OBJECTIVE:To explore the impact of different energy flow densities and numbers of shots of extracorporeal shock waves on the new vessel formation ability, migration capability and apoptosis of bone microvascular endothelial cel s. <br> METHODS:Bone microvascular endothelial cel s isolated from the femoral head of patients undergoing arthroplasty were subcultured in vitro, and then were immunofluorescently evaluated with endothelial cel marker antibodies to CD31 and von Wil ebrand factor (vWF), and grouped according to different energy flow densities (low, 0.03 mJ/mm2;high, 0.11 mJ/mm2) and numbers of shots (400 and 800). Capil ary-like tube formation, migration capability and apoptosis of bone microvascular endothelial cel s were determined by 3-D culture in vitro, scratch test, and flow cytometry, respectively. <br> RESULTS AND CONCLUSION:vWF and CD31 were positively expressed in approximately 100%of bone microvascular endothelial cel s, which indicates the cultured cel s had characterization of microvascular endothelial cel s. Extracorporeal shock wave enhanced angiogenesis and migration capability of bone microvascular endothelial cel s derived from the femoral head, and especial y low-energy flow density of extracorporeal shock wave exerted more superior effects. Angiogenesis of bone microvascular endothelial cel s was decreased with the increased shot number in the low-energy flow density group. In addition, extracorporeal shock wave inhibited bone microvascular endothelial cel apoptosis induced by steroids. Our results suggest that energy flow density and number of shots of extracorporeal shock waves impact the physiological function of bone microvascular endothelial cel s derived from the femoral head.关键词
组织构建/血管内皮细胞/体外震波/骨微血管内皮细胞/成管实验/划痕实验/流式细胞凋亡实验分类
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左伟,高福强,李配瑶,孙伟,李子荣,时令军..体外震波对骨微血管内皮细胞生理功能的影响[J].中国组织工程研究,2016,20(37):5504-5510,7.
