中国组织工程研究2016，Vol.20Issue(38)：5657-5663,7.DOI:10.3969/j.issn.2095-4344.2016.38.005
纳米级多孔负载人骨形成蛋白2基因修饰支架对前成骨细胞株分化的影响
Effects of nano porous beta-tricalcium phosphate/collagen scaffold modified with human bone morphogenetic protein 2 gene on differentiation of MC3T3-E1 cell lines
摘要
Abstract
BACKGROUND:Bone tissue transplantation or osteogenic material fil ing is after used for bone defect repair. To remove autologous bone tissues can lead to additional damage and secondary deformity, therefore, it is extremely urgent to search for a new osteogenic material. OBJECTIVE:To construct the porousβ-tricalcium phosphate (β-TCP)/col agen scaffold modified with human bone morphogenetic protein 2 (hBMP2) gene, and to observe its effects on differentiation of MC3T3-E1 cel lines. METHODS:The porousβ-TCP/col agen scaffold modified with hBMP2 gene was prepared. Then in vitro culture system of MC3T3-E1 cel lines with composite scaffold was established. There were scaffold and plate groups, and each group was divided into two subgroups according to the different concentrations of plasmid. Samples were col ected and observed morphological y by scanning electron microscope and light microscope after complex culture. After 1, 3, 7 and 14 days of induction, calcium nodules were observed through alizarin red staining, the cel cycle was detected by real-time PCR, and expressions ofαI-chain col agen type I gene, Osterix and bone sialoprotein were observed. RESULTS AND CONCLUSION:The number of cel s adhered, differentated and distributed on the composite scaffold was significantly higher than that of the single scaffold (P<0.05). Alizarin red staining and real-time PCR detection showed that the osteogenesis ability of MC3T3-E1 cel lines in the scaffold group was stronger than that in the plate group. To conclude, the porousβ-TCP/col agen scaffold modified with hBMP2 gene is an appropriate candidate for bone defect repair.关键词
生物材料/骨生物材料/人骨形成蛋白2/β-磷酸三钙/胶原/分化/骨缺损/黑龙江省自然科学基金分类
医药卫生引用本文复制引用
阮蔷,赵刚,郭睿,肖月,李超..纳米级多孔负载人骨形成蛋白2基因修饰支架对前成骨细胞株分化的影响[J].中国组织工程研究,2016,20(38):5657-5663,7.基金项目
黑龙江省自然科学基金项目(H201487)；佳木斯大学研究生创新项目(LZR2015_015) （H201487）
