摘要
Abstract
BACKGROUND:It has been found that the compressive strength and flexural strength of nano-hydroxyapatite combined with light-cured resin matrix can neet the requirements of dental restorative materials. OBJECTIVE:To detect the cytotoxicity of nano-hydroxyapatite/composite resin, and to analyze its effects on the dental restoration. METHODS:In vitro test:passage 5 periodontal ligament fibroblast-like cel s and L-929 cel s suspensions were cultured in the 10%, 50%, 100%of nano-hydroxyapatite/composite resin extracts, respectively, and fresh maintenance medium functioned as negative control group. Absorbance values were detected within 7-day culture. In vivo repair test:57 cases with tooth defect were enrol ed containing 37 males and 20 females aged from 15 to 31 years old, and al were subjected to nano-hydroxyapatite/composite resin treatment. A 3-year fol ow-up was undergone to detect the completeness, marginal adaptation and color matching of the restoration, as wel as the periodontal probing depth, sulcus bleeding index, tooth mobility, dental plaque index, gingival crevicular fluid and aspartate aminotransferase level. RESULTS AND CONCLUSION:In vitro test results showed that the different concentrations of nano-hydroxyapatite/composite resin extracts had no effects on both periodontal ligament fibroblast-like cel s and L-929 cel s. In vivo repair test results found that after 3 years of fol ow-up, the periodontal probing depth, sulcus bleeding index, tooth mobility, dental plaque index, gingival crevicular fluid and aspartate aminotransferase level of patients were significantly decreased than those before repair (P<0.05), but the alkaline phosphatase level revealed no significant change (P>0.05). Unfortunately, three cases with incomplete restoration, four cases with color mismatching, and three cases with the inadaptable edge occurred. To conclude, the nano-hydroxyapatite/composite resin holds good biocompatibility and no cytotoxicity, which can maintain a good periodontal health condition in the dental restoration.关键词
生物材料/纳米材料/纳米羟基磷灰石/复合树脂/牙体修复/细胞毒性/生物相容性/应用价值分类
医药卫生
